DC Field | Value | Language |
---|---|---|
dc.contributor.author | Singh, Raushan Kumar | ko |
dc.contributor.author | Singh, Ranjitha | ko |
dc.contributor.author | Sivakumar, Dakshinamurthy | ko |
dc.contributor.author | Kondaveeti, Sanath | ko |
dc.contributor.author | Kim, Taedoo | ko |
dc.contributor.author | Li, Jinglin | ko |
dc.contributor.author | Sung, Bong Hyun | ko |
dc.contributor.author | Cho, Byung-Kwan | ko |
dc.contributor.author | Kim, Dong Rip | ko |
dc.contributor.author | Kim, Sun Chang | ko |
dc.contributor.author | Kalia, Vipin C. | ko |
dc.contributor.author | Zhang, Yi-Heng P. Job | ko |
dc.contributor.author | Zhao, Huimin | ko |
dc.contributor.author | Kang, Yun Chan | ko |
dc.contributor.author | Lee, Jung-Kul | ko |
dc.date.accessioned | 2019-01-23T06:55:49Z | - |
dc.date.available | 2019-01-23T06:55:49Z | - |
dc.date.created | 2019-01-14 | - |
dc.date.issued | 2018-12 | - |
dc.identifier.citation | ACS CATALYSIS, v.8, no.12, pp.11085 - 11093 | - |
dc.identifier.issn | 2155-5435 | - |
dc.identifier.uri | http://hdl.handle.net/10203/250137 | - |
dc.description.abstract | Multienzymatic cascade reactions have garnered the attention of many researchers as an approach for converting CO, into methanol. The cascade reaction used in this study includes the following enzymes: a formate dehydrogenase (CIFDH), a formaldehyde dehydrogenase (BmFaldDH), and an alcohol dehydrogenase (YADH) from Clostridium ljungdahlii, Burkholderia multivorans, and Saccharomyces cerevisiae, respectively. Because this cascade reaction requires NADH as a cofactor, phosphite dehydrogenase (PTDH) was employed to regenerate the cofactor. The multienzymatic cascade reaction, along with PTDH, yielded 3.28 mM methanol. The key to the success of this cascade reaction was a novel formaldehyde dehydrogenase, BmFaldDH, the enzyme catalyzing the reduction of formate to formaldehyde. The methanol yield was further improved by incorporation of 1-ethyl-3-methylimidazolium acetate (EMIM-Ac), resulting in 7.86 mM of methanol. A 500-fold increase in total turnover number was observed for the CIFDH-BmFaldDH-YADH cascade system compared to the Candida boidinii FDH-Pseudomonas putida FaldDH-YADH system. We provided detailed insights into the enzymatic reduction of CO2 by determining the thermodynamic parameters (K-d and Delta G) using isothermal titration calorimetry. Furthermore, we demonstrated a novel time-dependent formaldehyde production from CO2. Our results will aid in the understanding and development of a robust multienzyme catalyzed cascade reaction for the reduction of CO2 to value-added chemicals. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Insights into Cell-Free Conversion of CO2 to Chemicals by a Multienzyme Cascade Reaction | - |
dc.type | Article | - |
dc.identifier.wosid | 000453491100014 | - |
dc.identifier.scopusid | 2-s2.0-85058292332 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.issue | 12 | - |
dc.citation.beginningpage | 11085 | - |
dc.citation.endingpage | 11093 | - |
dc.citation.publicationname | ACS CATALYSIS | - |
dc.identifier.doi | 10.1021/acscatal.8b02646 | - |
dc.contributor.localauthor | Cho, Byung-Kwan | - |
dc.contributor.localauthor | Kim, Sun Chang | - |
dc.contributor.nonIdAuthor | Singh, Raushan Kumar | - |
dc.contributor.nonIdAuthor | Singh, Ranjitha | - |
dc.contributor.nonIdAuthor | Sivakumar, Dakshinamurthy | - |
dc.contributor.nonIdAuthor | Kondaveeti, Sanath | - |
dc.contributor.nonIdAuthor | Kim, Taedoo | - |
dc.contributor.nonIdAuthor | Li, Jinglin | - |
dc.contributor.nonIdAuthor | Sung, Bong Hyun | - |
dc.contributor.nonIdAuthor | Kim, Dong Rip | - |
dc.contributor.nonIdAuthor | Kalia, Vipin C. | - |
dc.contributor.nonIdAuthor | Zhang, Yi-Heng P. Job | - |
dc.contributor.nonIdAuthor | Zhao, Huimin | - |
dc.contributor.nonIdAuthor | Kang, Yun Chan | - |
dc.contributor.nonIdAuthor | Lee, Jung-Kul | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
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