Multiplexed Metabolic Labeling of Glycoconjugates in Polarized Primary Cerebral Cortical Neurons

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The spatial distribution of cell-surface glycoconjugates in the brain changes continuously, reflecting neurophysiology especially in the developing phase, but their functions and fates mostly remain unexplored. Their spatiotemporal distribution is particularly important in polarized neuronal cells, such as cerebral cortical neurons composed of a soma and neurites. In this work, we dually labeled sialic acid (Sia5Ac) and N-acetylgalactosamine/glucosamine (GalNAc/GlcNAc) by a neurocompatible strategy of metabolic glycan labeling, metabolism-by-tissues (MbT), and obtained the multiplexed information on their spatiotemporal distribution on polarized cortical neurons. The analyses showed the preferentially distinct distribution of each saccharide set at the late developmental stage after randomized, heterogeneous distribution at the early stage, suggesting that Sia5Ac and GalNAc/GlcNAc are translocated anisotropically during neuronal development.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2018-11
Language
English
Article Type
Article
Citation

CHEMISTRY-AN ASIAN JOURNAL, v.13, no.22, pp.3480 - 3484

ISSN
1861-4728
DOI
10.1002/asia.201800996
URI
http://hdl.handle.net/10203/248287
Appears in Collection
CH-Journal Papers(저널논문)
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