DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chun, Jinyoung | ko |
dc.contributor.author | Seo, Sang Woo | ko |
dc.contributor.author | Jung, Gyoo Yeol | ko |
dc.contributor.author | Lee, Jinwoo | ko |
dc.date.accessioned | 2018-08-20T08:25:02Z | - |
dc.date.available | 2018-08-20T08:25:02Z | - |
dc.date.created | 2018-08-08 | - |
dc.date.created | 2018-08-08 | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | JOURNAL OF MATERIALS CHEMISTRY, v.21, no.18, pp.6713 - 6717 | - |
dc.identifier.issn | 0959-9428 | - |
dc.identifier.uri | http://hdl.handle.net/10203/245104 | - |
dc.description.abstract | The simple and efficient separation of histidine-tagged (His-tagged) proteins using nickel ferrite (NiFe(2)O(4)) nanoparticle clusters (NPCs) is described in this article. While nanostructured materials containing Ni(2+) ions for efficient separation were generally synthesized via complex synthetic procedures, the NiFe(2)O(4) nanoparticle clusters used in this work were simply synthesized by a one-pot hydrothermal polyol process. Their large surface area (105.0 m(2) g(-1)) and pore volume (0.32 cm(3) g(-1)) provide sufficient sites for the separation of His-tagged proteins. The high magnetic saturation value (41.3 emu g(-1)) and superparamagnetic property of these nanoparticle clusters lead to a more efficient magnetically recyclable separation of His-tagged proteins. We confirmed that the binding capacity and selective separation ability seen in the first separation were strongly maintained for up to 7 cycles. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | PURIFICATION METHOD | - |
dc.subject | SINGLE-CRYSTAL | - |
dc.subject | MICROSPHERES | - |
dc.subject | PEPTIDES | - |
dc.subject | NANORODS | - |
dc.subject | REMOVAL | - |
dc.subject | AGENT | - |
dc.subject | SHELL | - |
dc.subject | BIND | - |
dc.title | Easy access to efficient magnetically recyclable separation of histidine-tagged proteins using superparamagnetic nickel ferrite nanoparticle clusters | - |
dc.type | Article | - |
dc.identifier.wosid | 000289692500041 | - |
dc.identifier.scopusid | 2-s2.0-79955055264 | - |
dc.type.rims | ART | - |
dc.citation.volume | 21 | - |
dc.citation.issue | 18 | - |
dc.citation.beginningpage | 6713 | - |
dc.citation.endingpage | 6717 | - |
dc.citation.publicationname | JOURNAL OF MATERIALS CHEMISTRY | - |
dc.identifier.doi | 10.1039/c0jm04089d | - |
dc.contributor.localauthor | Lee, Jinwoo | - |
dc.contributor.nonIdAuthor | Chun, Jinyoung | - |
dc.contributor.nonIdAuthor | Seo, Sang Woo | - |
dc.contributor.nonIdAuthor | Jung, Gyoo Yeol | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PURIFICATION METHOD | - |
dc.subject.keywordPlus | SINGLE-CRYSTAL | - |
dc.subject.keywordPlus | MICROSPHERES | - |
dc.subject.keywordPlus | PEPTIDES | - |
dc.subject.keywordPlus | NANORODS | - |
dc.subject.keywordPlus | REMOVAL | - |
dc.subject.keywordPlus | AGENT | - |
dc.subject.keywordPlus | SHELL | - |
dc.subject.keywordPlus | BIND | - |
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