Development of rapid point-of-care assays for diagnosis of Gram-positive bacterial infections = 그람양성균 감염의 신속한 현장진단법 개발

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Development of a rapid, point-of-care assay for detecting bacterial pathogens is crucial to reduce the overuse of antibiotics and the spread of multidrug resistance. Herein, we describe a rapid, colorimetric assay based on the precipitation of nanobeads in the presence of Gram-positive bacteria. Vancomycin was immobilized onto colored polystyrene nanobeads to induce specific and multivalent binding with the Gram-positive bacterial cell wall and subsequent agglomeration. Without any pre-processing steps, the addition of various types of Gram-positive pathogens to the nanobeads resulted in the formation of blue precipitates, which could be observed with the naked eye in ~30 min. We also utilize a porous filter system to the assay, which allowed more clear discrimination of the positive signals of the formed precipitates, by reducing background signals from nonspecific agglomeration. We anticipate that the colorimetric nanobead assay can be potentially applied as a rapid and simple sensing platform, and enable point-of-care diagnosis of Gram-positive infections.
Advisors
Chung, Hyun Jungresearcher정현정researcher
Description
한국과학기술원 :나노과학기술대학원,
Publisher
한국과학기술원
Issue Date
2017
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 나노과학기술대학원, 2017.8,[iii, 42 p. :]

Keywords

Colorimetric assay▼aGram-positive bacteria▼aNanobeads▼aAgglomeration▼aPoint-of-care; 그람양성균▼a비색분석법▼a나노입자▼a감염진단▼a침전

URI
http://hdl.handle.net/10203/242914
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=718565&flag=dissertation
Appears in Collection
NT-Theses_Master(석사논문)
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