The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3

Cited 2 time in webofscience Cited 0 time in scopus
  • Hit : 234
  • Download : 266
The centromere is the chromosomal locus at which the kinetochore is assembled to direct chromosome segregation. The histone H3 variant, centromere protein A (CENP-A), is known to epigenetically mark active centromeres, but the mechanism by which CENP-A propagates at the centromere, replacing histone H3, remains poorly understood. Using fission yeast, here we show that the Ino80 adenosine triphosphate (ATP)-dependent chromatin-remodeling complex, which removes histone H3-containing nucleosomes from associated chromatin, promotes CENP-ACnp1 chromatin assembly at the centromere in a redundant manner with another chromatin-remodeling factor Chd1Hrp1. CENP-ACnp1 chromatin actively recruits the Ino80 complex to centromeres to elicit eviction of histone H3-containing nucleosomes. Artificial targeting of Ino80 subunits to a non-centromeric DNA sequence placed in a native centromere enhances the spreading of CENP-ACnp1 chromatin into the non-centromeric DNA. Based on these results, we propose that CENP-ACnp1 chromatin employs the Ino80 complex to mediate the replacement of histone H3 with CENP-ACnp1, and thereby reinforces itself.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2017-09
Language
English
Article Type
Article
Keywords

CHROMATIN REMODELING COMPLEX; CENP-A CHROMATIN; FISSION YEAST CENTROMERES; IN-VIVO; NUCLEOSOMES; CHROMOSOME; PROTEIN; HJURP; SCM3; DEPOSITION

Citation

NATURE COMMUNICATIONS, v.8

ISSN
2041-1723
DOI
10.1038/s41467-017-00704-3
URI
http://hdl.handle.net/10203/226284
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
Nat Communi 2017b.pdf(3.6 MB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 2 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0