DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seo, Sang Woo | ko |
dc.contributor.author | Gao, Ye | ko |
dc.contributor.author | Kim, Donghyuk | ko |
dc.contributor.author | Szubin, Richard | ko |
dc.contributor.author | Yang, Jina | ko |
dc.contributor.author | Cho, Byung-Kwan | ko |
dc.contributor.author | Palsson, Bernhard O. | ko |
dc.date.accessioned | 2017-06-16T04:01:12Z | - |
dc.date.available | 2017-06-16T04:01:12Z | - |
dc.date.created | 2017-06-05 | - |
dc.date.created | 2017-06-05 | - |
dc.date.created | 2017-06-05 | - |
dc.date.issued | 2017-05 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v.7 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10203/224076 | - |
dc.description.abstract | A transcription factor (TF), OmpR, plays a critical role in transcriptional regulation of the osmotic stress response in bacteria. Here, we reveal a genome-scale OmpR regulon in Escherichia coli K-12 MG1655. Integrative data analysis reveals that a total of 37 genes in 24 transcription units (TUs) belong to OmpR regulon. Among them, 26 genes show more than two-fold changes in expression level in an OmpR knock-out strain. Specifically, we find that: 1) OmpR regulates mostly membrane-located gene products involved in diverse fundamental biological processes, such as narU (encoding nitrate/nitrite transporter), ompX (encoding outer membrane protein X), and nuoN (encoding NADH: ubiquinone oxidoreductase); 2) by investigating co-regulation of entire sets of genes regulated by other stressresponse TFs, stresses are surprisingly independently regulated among each other; and, 3) a detailed investigation of the physiological roles of the newly discovered OmpR regulon genes reveals that activation of narU represents a novel strategy to significantly improve osmotic stress tolerance of E. coli. Thus, the genome-scale approach to elucidating regulons comprehensively identifies regulated genes and leads to fundamental discoveries related to stress responses. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | CHIP-SEQ DATA | - |
dc.subject | NEGATIVE REGULATION | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | GENE | - |
dc.subject | PROTEIN | - |
dc.subject | OSMOLARITY | - |
dc.subject | EXPRESSION | - |
dc.subject | NETWORK | - |
dc.subject | SYSTEM | - |
dc.subject | RNA | - |
dc.title | Revealing genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles under osmotic stress in Escherichia coli K-12 MG1655 | - |
dc.type | Article | - |
dc.identifier.wosid | 000401614900039 | - |
dc.identifier.scopusid | 2-s2.0-85019926080 | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.publicationname | SCIENTIFIC REPORTS | - |
dc.identifier.doi | 10.1038/s41598-017-02110-7 | - |
dc.contributor.localauthor | Cho, Byung-Kwan | - |
dc.contributor.nonIdAuthor | Seo, Sang Woo | - |
dc.contributor.nonIdAuthor | Gao, Ye | - |
dc.contributor.nonIdAuthor | Kim, Donghyuk | - |
dc.contributor.nonIdAuthor | Szubin, Richard | - |
dc.contributor.nonIdAuthor | Yang, Jina | - |
dc.contributor.nonIdAuthor | Palsson, Bernhard O. | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CHIP-SEQ DATA | - |
dc.subject.keywordPlus | NEGATIVE REGULATION | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | OSMOLARITY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | NETWORK | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | CHIP-SEQ DATA | - |
dc.subject.keywordPlus | NEGATIVE REGULATION | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | OSMOLARITY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | NETWORK | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | RNA | - |
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