DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Kwang-Hyun | ko |
dc.contributor.author | An, Yan | ko |
dc.contributor.author | Jung, Tae Yang | ko |
dc.contributor.author | Baek, In-Young | ko |
dc.contributor.author | Noh, Haemin | ko |
dc.contributor.author | Ahn, Woo-Chan | ko |
dc.contributor.author | Hebert, Hans | ko |
dc.contributor.author | Song, Ji-Joon | ko |
dc.contributor.author | Kim, Jeong-Hoon | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.contributor.author | Woo, Eui-Jeon | ko |
dc.date.accessioned | 2017-06-05T02:20:27Z | - |
dc.date.available | 2017-06-05T02:20:27Z | - |
dc.date.created | 2017-05-29 | - |
dc.date.created | 2017-05-29 | - |
dc.date.issued | 2017-05 | - |
dc.identifier.citation | EMBO REPORTS, v.18, no.5, pp.826 - 840 | - |
dc.identifier.issn | 1469-221X | - |
dc.identifier.uri | http://hdl.handle.net/10203/223910 | - |
dc.description.abstract | The CRISPR-Cas system is an adaptive and heritable immune response that destroys invading foreign nucleic acids. The effector complex of the Type III CRISPR-Cas system targets RNA and DNA in a transcription-coupled manner, but the exact mechanism of DNA targeting by this complex remains elusive. In this study, an effector Csm holocomplex derived from Thermococcus onnurineus is reconstituted with a minimalistic combination of Csm1(1)2(1)3(3)4(1)5(1), and shows RNA targeting and RNA-activated single-stranded DNA (ssDNA) targeting activities. Unexpectedly, in the absence of an RNA transcript, it cleaves ssDNA containing a sequence complementary to the bound crRNA guide region in a manner dependent on the HD domain of the Csm1 subunit. This nuclease activity is blocked by a repeat tag found in the host CRISPR loci. The specific cleavage of ssDNA without a target RNA suggests a novel ssDNA targeting mechanism of the Type III system, which could facilitate the efficient and complete degradation of foreign nucleic acids. | - |
dc.language | English | - |
dc.publisher | WILEY | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | EFFECTOR COMPLEX | - |
dc.subject | STRUCTURAL BASIS | - |
dc.subject | IMMUNE-SYSTEM | - |
dc.subject | CMR COMPLEX | - |
dc.subject | CLEAVAGE | - |
dc.subject | RECOGNITION | - |
dc.subject | CASCADE | - |
dc.subject | INTERFERENCE | - |
dc.subject | PROKARYOTES | - |
dc.title | RNA activation-independent DNA targeting of the Type III CRISPR-Cas system by a Csm complex | - |
dc.type | Article | - |
dc.identifier.wosid | 000400446100016 | - |
dc.identifier.scopusid | 2-s2.0-85017406426 | - |
dc.type.rims | ART | - |
dc.citation.volume | 18 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 826 | - |
dc.citation.endingpage | 840 | - |
dc.citation.publicationname | EMBO REPORTS | - |
dc.identifier.doi | 10.15252/embr.201643700 | - |
dc.contributor.localauthor | Song, Ji-Joon | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Park, Kwang-Hyun | - |
dc.contributor.nonIdAuthor | An, Yan | - |
dc.contributor.nonIdAuthor | Baek, In-Young | - |
dc.contributor.nonIdAuthor | Hebert, Hans | - |
dc.contributor.nonIdAuthor | Kim, Jeong-Hoon | - |
dc.contributor.nonIdAuthor | Woo, Eui-Jeon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CRISPR | - |
dc.subject.keywordAuthor | Csm complex | - |
dc.subject.keywordAuthor | DNase | - |
dc.subject.keywordAuthor | RNase | - |
dc.subject.keywordAuthor | Thermococcus onnurineus | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | EFFECTOR COMPLEX | - |
dc.subject.keywordPlus | STRUCTURAL BASIS | - |
dc.subject.keywordPlus | IMMUNE-SYSTEM | - |
dc.subject.keywordPlus | CMR COMPLEX | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | CASCADE | - |
dc.subject.keywordPlus | INTERFERENCE | - |
dc.subject.keywordPlus | PROKARYOTES | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.