A mass spectrometry-based multiplex SNP genotyping by utilizing allele specific ligation and strand displacement amplification

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dc.contributor.authorPark, Jung-Hunko
dc.contributor.authorJang, Hyowonko
dc.contributor.authorJung, Yun Kyungko
dc.contributor.authorJung, Ye Limko
dc.contributor.authorShin, Inkyungko
dc.contributor.authorCho, Dae-Yeonko
dc.contributor.authorPark, Hyun Gyuko
dc.date.accessioned2017-05-10T04:00:50Z-
dc.date.available2017-05-10T04:00:50Z-
dc.date.created2016-11-30-
dc.date.created2016-11-30-
dc.date.issued2017-05-
dc.identifier.citationBIOSENSORS & BIOELECTRONICS, v.91, pp.122 - 127-
dc.identifier.issn0956-5663-
dc.identifier.urihttp://hdl.handle.net/10203/223579-
dc.description.abstractWe herein describe a new mass spectrometry-based method for multiplex SNP genotyping by utilizing allele specific ligation and strand displacement amplification (SDA) reaction. In this method, allele-specific ligation is first performed to discriminate base sequence variations at the SNP site within the PCR-amplified target DNA. The primary ligation probe is extended by a universal primer annealing site while the secondary ligation probe has base sequences as an overhang with a nicking enzyme recognition site and complementary mass marker sequence. The ligation probe pairs are ligated by DNA ligase only at specific allele in the target DNA and the resulting ligated product serves as a template to promote the SDA reaction using a universal primer. This process isothermally amplifies short DNA fragments, called mass markers, to be analyzed by mass spectrometry. By varying the sizes of the mass markers, we successfully demonstrated the multiplex SNP genotyping capability of this method by reliably identifying several BRCA mutations in a multiplex manner with mass spectrometry.-
dc.languageEnglish-
dc.publisherELSEVIER ADVANCED TECHNOLOGY-
dc.subjectSINGLE-NUCLEOTIDE POLYMORPHISMS-
dc.subjectENHANCED RAMAN-SPECTROSCOPY-
dc.subjectPROBE AMPLIFICATION-
dc.subjectCOMPLEX DISEASES-
dc.subjectHUMAN GENOME-
dc.subjectDNA-
dc.subjectBRCA1-
dc.subjectMUTATIONS-
dc.subjectBIOSENSOR-
dc.subjectSURFACE-
dc.titleA mass spectrometry-based multiplex SNP genotyping by utilizing allele specific ligation and strand displacement amplification-
dc.typeArticle-
dc.identifier.wosid000395599000019-
dc.identifier.scopusid2-s2.0-85007168367-
dc.type.rimsART-
dc.citation.volume91-
dc.citation.beginningpage122-
dc.citation.endingpage127-
dc.citation.publicationnameBIOSENSORS & BIOELECTRONICS-
dc.identifier.doi10.1016/j.bios.2016.10.065-
dc.contributor.localauthorPark, Hyun Gyu-
dc.contributor.nonIdAuthorShin, Inkyung-
dc.contributor.nonIdAuthorCho, Dae-Yeon-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorMass spectrometry-
dc.subject.keywordAuthorSNP genotyping-
dc.subject.keywordAuthorBRCA gene-
dc.subject.keywordAuthorAllele-specific ligation-
dc.subject.keywordAuthorStrand displacement amplification-
dc.subject.keywordAuthorLabel-free biosensor-
dc.subject.keywordPlusSINGLE-NUCLEOTIDE POLYMORPHISMS-
dc.subject.keywordPlusENHANCED RAMAN-SPECTROSCOPY-
dc.subject.keywordPlusPROBE AMPLIFICATION-
dc.subject.keywordPlusCOMPLEX DISEASES-
dc.subject.keywordPlusHUMAN GENOME-
dc.subject.keywordPlusDNA-
dc.subject.keywordPlusBRCA1-
dc.subject.keywordPlusMUTATIONS-
dc.subject.keywordPlusBIOSENSOR-
dc.subject.keywordPlusSURFACE-
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