DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chae, Cheol Gi | ko |
dc.contributor.author | Kim, You Jin | ko |
dc.contributor.author | Lee, Se Jin | ko |
dc.contributor.author | Oh, Young Hoon | ko |
dc.contributor.author | Yang, Jung Eun | ko |
dc.contributor.author | Joo, Jeong Chan | ko |
dc.contributor.author | Kang, Kyoung Hee | ko |
dc.contributor.author | Jang, Young-Ah | ko |
dc.contributor.author | Lee, Hyuk | ko |
dc.contributor.author | Park, A-Reum | ko |
dc.contributor.author | Song, Bong Keun | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.contributor.author | Park, Si Jae | ko |
dc.date.accessioned | 2016-06-30T00:36:50Z | - |
dc.date.available | 2016-06-30T00:36:50Z | - |
dc.date.created | 2016-04-19 | - |
dc.date.created | 2016-04-19 | - |
dc.date.created | 2016-04-19 | - |
dc.date.issued | 2016-01 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.21, no.1, pp.169 - 174 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | http://hdl.handle.net/10203/208684 | - |
dc.description.abstract | We have previously reported in vivo biosynthesis of polyhydroxyalkanoates containing 2-hydroxyacid monomers such as lactate and 2-hydroxybutyrate in recombinant Escherichia coli strains by the expression of evolved Clostridium propionicum propionyl-CoA transferase (PctCp) and Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate (PHA) synthase 1 (PhaC1 (Ps6-19)). Here, we report the biosynthesis of poly(2-hydroxybutyrate-co-lactate)[P(2HB-co-LA)] by direct fermentation of metabolically engineered E. coli strain. Among E. coli strains WL3110, XL1-Blue, and BL21(DE3), recombinant E. coli XL1-Blue strain expressing PhaC1437 and Pct540 produced P(76.4mol%2HB-co-23.6mol%LA) to the highest content of 88 wt% when it was cultured in a chemically defined medium containing 20 g/L of glucose and 2 g/L of sodium 2-hydroxybutyrate. When recombinant E. coli XL1-Blue strain expressing PhaC1437 and Pct540 was cultured in a chemically defined medium containing 20 g/L of glucose and varying concentration of sodium 2-hydroxybutyrate, 2HB monomer fraction in P(2HB-co-LA) increased proportional to the concentration of sodium 2-hydroxybutyrate added to the culture medium. P(2HB-co-LA)] could also be produced from glucose as a sole carbon source without sodium 2-hydroxybutyrate into the culture medium. Recombinant E. coli XL1-Blue strain expressing the phaC1437, pct540, cimA3.7, and leuBCD genes together with the L. lactis Il1403 panE gene, successfully produced P(23.5mol%2HB-co-76.5mol%LA)] to the polymer content of 19.4 wt% when it cultured in a chemically defined medium containing 20 g/L of glucose. The metabolic engineering strategy reported here should be useful for the production of novel copolymer P(2HB-co-LA)] | - |
dc.language | English | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Biosynthesis of poly(2-hydroxybutyrate-co-lactate) in metabolically engineered Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000372595700019 | - |
dc.identifier.scopusid | 2-s2.0-84961258740 | - |
dc.type.rims | ART | - |
dc.citation.volume | 21 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 169 | - |
dc.citation.endingpage | 174 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.identifier.doi | 10.1007/s12257-015-0749-x | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.nonIdAuthor | Chae, Cheol Gi | - |
dc.contributor.nonIdAuthor | Kim, You Jin | - |
dc.contributor.nonIdAuthor | Lee, Se Jin | - |
dc.contributor.nonIdAuthor | Oh, Young Hoon | - |
dc.contributor.nonIdAuthor | Joo, Jeong Chan | - |
dc.contributor.nonIdAuthor | Kang, Kyoung Hee | - |
dc.contributor.nonIdAuthor | Jang, Young-Ah | - |
dc.contributor.nonIdAuthor | Lee, Hyuk | - |
dc.contributor.nonIdAuthor | Park, A-Reum | - |
dc.contributor.nonIdAuthor | Song, Bong Keun | - |
dc.contributor.nonIdAuthor | Park, Si Jae | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | PHA | - |
dc.subject.keywordAuthor | 2-hydroxyacid | - |
dc.subject.keywordAuthor | lactate | - |
dc.subject.keywordAuthor | 2-hydroxybutyrate | - |
dc.subject.keywordAuthor | poly(2-hydroxybutyrate-co-lactate) | - |
dc.subject.keywordAuthor | recombinant E. coli | - |
dc.subject.keywordPlus | LACTATE-CONTAINING POLYESTERS | - |
dc.subject.keywordPlus | BACTERIAL POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | MICROBIAL-PRODUCTION | - |
dc.subject.keywordPlus | RALSTONIA-EUTROPHA | - |
dc.subject.keywordPlus | POLYLACTIC ACID | - |
dc.subject.keywordPlus | 2-HYDROXYBUTYRATE | - |
dc.subject.keywordPlus | COPOLYMERS | - |
dc.subject.keywordPlus | SYNTHASES | - |
dc.subject.keywordPlus | CHEMICALS | - |
dc.subject.keywordPlus | BIOMASS | - |
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