DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, TJ | ko |
dc.contributor.author | Park, JP | ko |
dc.contributor.author | Lee, SJ | ko |
dc.contributor.author | Hong, HJ | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-12-09T01:09:52Z | - |
dc.date.available | 2010-12-09T01:09:52Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-03 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.11, no.2, pp.173 - 177 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20864 | - |
dc.description.abstract | In this study, a novel strategy was developed for the highly selective immobilization of proteins, using the polyhydroxyalkanoate (PHA) depolymerase substrate binding domain (SBD) as an active binding domain. In order to determine the appropriacy of this method for immunodiagnostic assays, the single-chain antibody (ScFv) against the hepatitis B virus (HBV) preS2 surface protein and the severe acute respiratory syndrome coronavirus (SARS-CoV) envelope protein (SCVe) were fused to the SBD, then directly immobilized on PHA-coated slides via microspotting. The fluorescence-labeled HBV antigen and the antibody against SCVe were then utilized to examine specific interactions on the PHA-coated surfaces. Fluorescence signals were detected only at the spotted positions, thereby indicating a high degree of affinity and selectivity for their corresponding antigens/antibodies. Furthermore, we detected small amounts of ScFv-SBD (2.7 ng/mL) and SCVe-SBD fusion proteins (0.6 ng/mL). Therefore, this microarray platform technology, using PHA and SBD, appears generally appropriate for immunodiagnosis, with no special requirements with regard to synthetic or chemical modification of the biomolecules or the solid surface. | - |
dc.description.sponsorship | We would like to thank Dr.Lee, J.-J at LG Life Sciences for generously providing us with HBV preS2 surface protein. This work was supported by KOSEF through the Center for Ultramicrochemical Process Systems. Further support given by the LG Chem Chair Professorship and the BK21 program are alos greatly appreciated. It should be mentioned that some of the results reported in this paper have been included in the following invited book chapter review, which is slated to be published in the future [Microbial Bionanotechnology: Biological Self-Assembly Systems and Biopolymer-Based Nanostructures, B. Rehm (ed.), Horizon Bioscience, Norwich, UK, 2006]. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Polyhydroxyalkanoate chip for the specific immobilization of recombinant proteins and its applications in immunodiagnostics | - |
dc.type | Article | - |
dc.identifier.wosid | 000237309900016 | - |
dc.identifier.scopusid | 2-s2.0-33646446464 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 173 | - |
dc.citation.endingpage | 177 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.identifier.doi | 10.1007/BF02931904 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Park, TJ | - |
dc.contributor.nonIdAuthor | Park, JP | - |
dc.contributor.nonIdAuthor | Lee, SJ | - |
dc.contributor.nonIdAuthor | Hong, HJ | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | poly(3-hydroxybutyrate) | - |
dc.subject.keywordAuthor | microarray | - |
dc.subject.keywordAuthor | HBV preS2 surface protein | - |
dc.subject.keywordAuthor | SARS-CoV envelope protein | - |
dc.subject.keywordAuthor | P(3HB) depolymerase substrate binding domain | - |
dc.subject.keywordPlus | HEPATITIS-B-VIRUS | - |
dc.subject.keywordPlus | RESPIRATORY SYNDROME CORONAVIRUS | - |
dc.subject.keywordPlus | SELECTIVE IMMOBILIZATION | - |
dc.subject.keywordPlus | ANTIBODY-RESPONSES | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | C VIRUS | - |
dc.subject.keywordPlus | MICROARRAYS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | TECHNOLOGY | - |
dc.subject.keywordPlus | ASSAY | - |
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