Solar-to-chemical conversion platform by Robust Cytochrome P450-P(3HB) complex

Abstract

Cytochrome P450 monooxygenases which catalyze a remarkable variety of oxidative transformation are of exceptional interest for the synthesis of fine chemicals. However, due to the instability and requirement of expensive cofactor, P450s have yet to be extensively used for industry. Here, we developed a new platform that combines a solar-driven cofactor regeneration and immobilization of P450 on biopolymer. Through photochemical cofactor regeneration using eosin Y as a photosensitizer, P450-P(3HB) complex successfully catalyzed a O-dealkylation reaction. Furthermore, using the P450-P(3HB) complex and a solar-tracking module, we achieved a P450-catalyzed reaction under natural sunlight for four consecutive days in a preparative scale.