Ubiquitination-dependent degradation of p73 by the mitochondrial E3 ubiquitin ligase Hades

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p73 is a member of the p53 family of transcription factors which plays an essential role in tumor suppression. p73 is associated with the sensitivity of cancer cells to chemotherapy and the prognosis of many cancers. In this study, we showed the ubiquitination-dependent degradation of p73 by the mitochondrial E3 ubiquitin ligase Hades. First, the binding between p73 and Hades was identified by coimmunoprecipitation experiments, and it was found that the Hades RING-finger domain mediates the interaction with p73. Immunofluorescence analysis showed that p73 moves to the mitochondria and colocalizes with Hades during etoposide-induced apoptosis. By performing in vivo and in vitro ubiquitination assays, we observed that the Hades RING-finger domain promotes ubiquitination of p73. Finally, it was shown that SiRNA-mediated depletion of Hades stabilizes p73. Taken together, our results showed that Hades mediates the ubiquitination-dependent degradation of mitochondrial p73 under apoptotic conditions. These findings suggest that Hades-mediated p73 ubiquitination is a novel regulatory mechanism for the exonuclear function of p73.
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Issue Date
2015-11
Language
English
Article Type
Article
Keywords

P53 PROTEIN; TUMOR-SUPPRESSOR; APOPTOSIS; TRANSACTIVATION; STABILITY; DOMAIN; TAP73; DELTA-NP73; MECHANISM; PATHWAY

Citation

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.467, no.2, pp.316 - 321

ISSN
0006-291X
DOI
10.1016/j.bbrc.2015.09.163
URI
http://hdl.handle.net/10203/207484
Appears in Collection
BiS-Journal Papers(저널논문)
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