A microfluidic array for large-scale ordering and orientation of embryos

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Quantitative studies of embryogenesis require the ability to monitor pattern formation and morphogenesis in large numbers of embryos, at multiple time points and in diverse genetic backgrounds. We describe a simple approach that greatly facilitates these tasks for Drosophila melanogaster embryos, one of the most advanced models of developmental genetics. Based on passive hydrodynamics, we developed a microfluidic embryo-trap array that can be used to rapidly order and vertically orient hundreds of embryos. We describe the physical principles of the design and used this platform to quantitatively analyze multiple morphogen gradients in the dorsoventral patterning system. Our approach can also be used for live imaging and, with slight modifications, could be adapted for studies of pattern formation and morphogenesis in other model organisms.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2011-02
Language
English
Article Type
Article
Keywords

DROSOPHILA-EMBRYO; DORSOVENTRAL PATTERN; DORSAL MORPHOGEN; GRADIENT; NUCLEAR; GENE; EXPRESSION; PROTEIN

Citation

NATURE METHODS, v.8, no.2, pp.171 - 103

ISSN
1548-7091
DOI
10.1038/NMETH.1548
URI
http://hdl.handle.net/10203/207335
Appears in Collection
CBE-Journal Papers(저널논문)
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