1-Propanol production from overproduced amino acid in metabolically engineered Escherichia coli

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1-propanol is considered as a promising new generation of biofuel that can substitute agasoline. In this study, we introduced a novel biosynthetic pathway toward 1-propanolusing L-threonine overproducing Escherichia coli as a base strain. The highest titer of1-propanol (3.5g/L) was achieved by Atsumi et al., using recombinant Escherichia coliharboring heterogenous gene encoding Methanococcus jannaschii citramalate synthase(CimA), which directly converts pyruvate into 2-ketobutyrate. The previously reportedE. coli TH20 which overproducing L-threonine was used as base strain in this study. Togenerate 1-propanol producing E. coli strain, competing pathways were deleted andcarbon source was optimized by in silico flux reponse analysis. Further metabolicengineering of the final strain improved the production rate moreover. [This work wassupported by the Advanced Biomass R&D Center of Korea through the Global Frontier Research Program of the Ministry of Education, Science andTechnology (MEST). Further supports by BioFuelChem, EEWS program of KAIST, andthe World Class University program of the MEST areappreciated.]
Publisher
한국과학공학회
Issue Date
2015-10-23
Language
English
Citation

2015 KIChE Fall Meeting, pp.1743 - 1743

ISSN
1225-9004
URI
http://hdl.handle.net/10203/205799
Appears in Collection
CBE-Conference Papers(학술회의논문)
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