DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Seongsu | ko |
dc.contributor.author | Kim, Kwon Ho | ko |
dc.contributor.author | Kim, Yeu-Chun | ko |
dc.date.accessioned | 2016-04-20T06:13:54Z | - |
dc.date.available | 2016-04-20T06:13:54Z | - |
dc.date.created | 2015-11-30 | - |
dc.date.created | 2015-11-30 | - |
dc.date.issued | 2015-11 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v.5 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10203/205186 | - |
dc.description.abstract | Electroporation is one of the most widely used transfection methods because of its high efficiency and convenience among the various transfection methods. Previous micro-electroporation systems have some drawbacks such as limitations in height and design, time-consuming and an expensive fabrication process due to technical constraints. This study fabricates a three dimensional microelectrode using the 3D printing technique. The interdigitated microstructure consisting of poly lactic acid was injected by a 3D printer and coated with silver and aluminum with a series of dip-coatings. With the same strength of electric field (V cm(-1)), a higher efficiency for molecular delivery and a higher cellular viability are achieved with the microelectrode than with a standard cuvette. In addition, this study investigates chemicophysical changes such as Joule heating and dissolved metal during electroporation and showed the micro-electroporation system had less chemicophysical changes. It was concluded that the proposed micro-electroporation system will contribute to genetic engineering as a promising delivery tool, and this combination of 3D printing and electroporation has many potential applications for diverse designs or systems. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | MICROFLUIDIC ELECTROPORATION | - |
dc.subject | NUCLEAR TRANSFORMATION | - |
dc.subject | CHEMICAL-SYNTHESIS | - |
dc.subject | GENE-THERAPY | - |
dc.subject | STEM-CELLS | - |
dc.subject | IN-VIVO | - |
dc.subject | TRANSFECTION | - |
dc.subject | DEVICE | - |
dc.subject | NANOPARTICLES | - |
dc.subject | REACTIONWARE | - |
dc.title | A novel electroporation system for efficient molecular delivery into Chlamydomonas reinhardtii with a 3-dimensional microelectrode | - |
dc.type | Article | - |
dc.identifier.wosid | 000363903700001 | - |
dc.identifier.scopusid | 2-s2.0-84946594693 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.publicationname | SCIENTIFIC REPORTS | - |
dc.identifier.doi | 10.1038/srep15835 | - |
dc.contributor.localauthor | Kim, Yeu-Chun | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MICROFLUIDIC ELECTROPORATION | - |
dc.subject.keywordPlus | NUCLEAR TRANSFORMATION | - |
dc.subject.keywordPlus | CHEMICAL-SYNTHESIS | - |
dc.subject.keywordPlus | GENE-THERAPY | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | DEVICE | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | REACTIONWARE | - |
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