생분해성 고분자, 폴리하이드록시알카노에이트를 이용한 바이오센서 칩 연구와 그 응용 Research and Application of Polyhydroxyalkanoates in Biosensor Chip

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Polyhydroxyalkanoates (PHAs) are a family of microbial polyesters that can be produced by fermentation from renewable resources. PHAs can be used as completely biodegradable plastics or elastomers. In this paper, novel applications of PHAs in biosensor are described. A general platform technology was developed by using the substrate binding domain (SBD) of PHA depolymerase as a fusion partner to immobilize proteins of interest on PHA surface. It could be shown that the proteins fused to the SBD of PHA depolymerase could be specifically immobilized onto PHA film, PHA microbead, and microcontact printed PHA surface. We review the results obtained for monitoring the specific interaction between the SBD and PHA by using enhanced green fluorescent protein, red fluorescent protein, single chain antibody against hepatitis B virus preS2 surface protein and severe acute respiratory syndrome coronavirus surface antigen as model proteins. Thus, this system can be efficiently used for studying protein-protein and possibly protein-biomolecule interactions for various biotechnological applications.
Publisher
한국생물공학회
Issue Date
2007
Keywords

Poly(3-hydroxybutyrate); P(3HB) depolymerase; substrate binding domain; protein microarray

Citation

Korean Journal of Biotechnology and Bioengineering, Vol.22, No.6, pp.371-377

URI
http://hdl.handle.net/10203/20326
Appears in Collection
CBE-Journal Papers(저널논문)

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