Transcript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli

The responses to phosphate starvation by PhoR-PhoB two-component regulatory system are also affected by other regulatory systems in Escherichia coli. The interactions among PhoB, PhoR, PhoU and CreC were investigated at transcript and protein levels using real-time PCR and fluorescence resonance energy transfer analyses. CreC showed an interaction with PhoB, before an interaction between PhoB and PhoR, suggesting that this acts as a constant sensor for the early response to phosphate starvation. PhoU was found to interact with neither PhoB nor PhoR, while this acted as a phosphate starvation-inducible protein. These results showed the detailed time- and phosphate concentration-dependent interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in E. coli.
Publisher
BLACKWELL PUBLISHING
Issue Date
2007-12
Language
ENG
Keywords

RESONANCE ENERGY-TRANSFER; LIVING CELLS; REGULON; OPERON

Citation

FEMS MICROBIOLOGY LETTERS, v.277, pp.254 - 259

ISSN
0378-1097
DOI
10.1111/j.1574-6968.2007.00965.x
URI
http://hdl.handle.net/10203/20273
Appears in Collection
CBE-Journal Papers(저널논문)
  • Hit : 106
  • Download : 0
  • Cited 0 times in thomson ci
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡClick to seewebofscience_button
⊙ Cited 10 items in WoSClick to see citing articles inrecords_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0