High cell-density culture of Escherichia coli

Cited 576 time in webofscience Cited 0 time in scopus
  • Hit : 190
  • Download : 55
Escherichia coli is the most widely used prokaryotic system for the synthesis of heterologous proteins. Once an optimal expression system has been constructed, protein production can be enhanced by increasing the production of protein per cell per unit time (specific productivity), or by increasing the cell concentration per unit time (cell productivity), Various high cell-density culture (HCDC) techniques have been developed for growing recombinant and non-recombinant E, coli strains in fed-batch cultures at concentrations greater than 100 grams (dry cell weight) per liter. This article reviews the problems encountered in HCDC of E. coli, and discusses various solutions, Feeding strategies for HCDC of E. coli, and the results obtained using them, are also described.
Publisher
ELSEVIER SCI LTD
Issue Date
1996-03
Language
English
Article Type
Article
Citation

TRENDS IN BIOTECHNOLOGY, v.14, no.3, pp.98 - 105

ISSN
0167-7799
URI
http://hdl.handle.net/10203/19895
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 576 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0