Whole Cell Bioconversion of Ricinoleic Acid to 12-Ketooleic Acid by Recombinant Corynebacterium glutamicum-Based Biocatalyst

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dc.contributor.authorLee, Byeonghunko
dc.contributor.authorLee, Saebomko
dc.contributor.authorKim, Hyeonsooko
dc.contributor.authorJeong, Kijunko
dc.contributor.authorPark, Jinbyungko
dc.contributor.authorPark, Kyungmoonko
dc.contributor.authorLee, Jinwonko
dc.date.accessioned2015-06-03T06:23:57Z-
dc.date.available2015-06-03T06:23:57Z-
dc.date.created2015-05-26-
dc.date.created2015-05-26-
dc.date.created2015-05-26-
dc.date.issued2015-04-
dc.identifier.citationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.25, no.4, pp.452 - 458-
dc.identifier.issn1017-7825-
dc.identifier.urihttp://hdl.handle.net/10203/198720-
dc.description.abstractThe biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35 degrees C, and 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; and 3.9 mM 12-ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicum-based biocatalyst.-
dc.languageEnglish-
dc.publisherKOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY-
dc.titleWhole Cell Bioconversion of Ricinoleic Acid to 12-Ketooleic Acid by Recombinant Corynebacterium glutamicum-Based Biocatalyst-
dc.typeArticle-
dc.identifier.wosid000353664000005-
dc.identifier.scopusid2-s2.0-84948688399-
dc.type.rimsART-
dc.citation.volume25-
dc.citation.issue4-
dc.citation.beginningpage452-
dc.citation.endingpage458-
dc.citation.publicationnameJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.identifier.doi10.4014/jmb.1501.01001-
dc.contributor.localauthorJeong, Kijun-
dc.contributor.nonIdAuthorLee, Byeonghun-
dc.contributor.nonIdAuthorLee, Saebom-
dc.contributor.nonIdAuthorKim, Hyeonsoo-
dc.contributor.nonIdAuthorPark, Jinbyung-
dc.contributor.nonIdAuthorPark, Kyungmoon-
dc.contributor.nonIdAuthorLee, Jinwon-
dc.type.journalArticleArticle-
dc.subject.keywordAuthor12-Ketooleic acid-
dc.subject.keywordAuthorricinoleic acid-
dc.subject.keywordAuthorsecondary alcohol dehydrogenase-
dc.subject.keywordAuthorwhole cell bioconversion-
dc.subject.keywordAuthorCorynebacterium glutamicum-
dc.subject.keywordPlusOLEIC-ACID-
dc.subject.keywordPlusFATTY-ACIDS-
dc.subject.keywordPlus10-KETOSTEARIC ACID-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusNRRL B-14859-
dc.subject.keywordPlusCONVERSION-
dc.subject.keywordPlusDETERGENTS-
dc.subject.keywordPlusEXPRESSION-
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CBE-Journal Papers(저널논문)
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