DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sun, Yujin | ko |
dc.contributor.author | Kim, Hoe Suk | ko |
dc.contributor.author | Park, Jinho | ko |
dc.contributor.author | Li, Mulan | ko |
dc.contributor.author | Tian, Lianji | ko |
dc.contributor.author | Choi, YoonSeok | ko |
dc.contributor.author | Choi, Byung Ihn | ko |
dc.contributor.author | Jon, Sangyong | ko |
dc.contributor.author | Moon, Woo Kyung | ko |
dc.date.accessioned | 2015-04-07T04:28:25Z | - |
dc.date.available | 2015-04-07T04:28:25Z | - |
dc.date.created | 2014-12-22 | - |
dc.date.created | 2014-12-22 | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | THERANOSTICS, v.4, no.8, pp.845 - 857 | - |
dc.identifier.issn | 1838-7640 | - |
dc.identifier.uri | http://hdl.handle.net/10203/195175 | - |
dc.description.abstract | The identification of breast tumor initiating cells (BTICs) is important for the diagnosis and therapy of breast cancers. This study was undertaken to evaluate whether the extra domain-B of fibronectin (EDB-FN) could be used as a new biomarker for BTICs and whether EDB-FN targeting superparamagnetic iron oxide nanoparticles (SPIONs) could be used as a magnetic resonance imaging (MRI) contrast agent for BTIC imaging in vitro and in vivo. BTICs (NDY-1) exhibited high EDB-FN expression, whereas non-BTICs (MCF-7, BT-474, SUM-225, MDA-MB-231) did not exhibit EDB-FN expression. Furthermore, Cy3.3-labeled EDB-FN specific peptides (APT(EDB)) showed preferential binding to the targeted NDY-1 cells. To construct an EDB-FN targeted imaging probe, APT(EDB) was covalently attached to a thermally cross-linked SPION (TCL-SPION) to yield APT(EDB)-TCL-SPION. In the in vitro MRI of cell phantoms, selective binding of APT(EDB)-TCL-SPION to NDY-1 cells was evident, but little binding was observed in MCF-7 cells. After the intravenous injection of APT(EDB)-TCL-SPION into the NDY-1 mouse tumor xenograft model, a significant decrease in the signal within the tumor was observed in the T-2*-weighted images; however, there was only a marginal change in the signal of non-targeting SPIONs such as APT(scramble)-TCL-SPION or TCL-SPION. Taken together, we report for the first time that EDB-FN was abundantly expressed in BTICs and may therefore be useful as a new biomarker for identifying BTICs. Our study also suggests that APT(EDB)-TCL-SPION could be used as an MRI contrast agent for BTIC imaging. | - |
dc.language | English | - |
dc.publisher | IVYSPRING INT PUBL | - |
dc.subject | SUPERPARAMAGNETIC IRON-OXIDE | - |
dc.subject | ORAL SQUAMOUS-CELL | - |
dc.subject | STEM-CELLS | - |
dc.subject | IN-VIVO | - |
dc.subject | CANCER | - |
dc.subject | THERAPY | - |
dc.subject | CARCINOMA | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | EXPRESSION | - |
dc.subject | MARKER | - |
dc.title | MRI of Breast Tumor Initiating Cells Using the Extra Domain-B of Fibronectin Targeting Nanoparticles | - |
dc.type | Article | - |
dc.identifier.wosid | 000345215100008 | - |
dc.identifier.scopusid | 2-s2.0-84903901410 | - |
dc.type.rims | ART | - |
dc.citation.volume | 4 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 845 | - |
dc.citation.endingpage | 857 | - |
dc.citation.publicationname | THERANOSTICS | - |
dc.identifier.doi | 10.7150/thno.8343 | - |
dc.contributor.localauthor | Jon, Sangyong | - |
dc.contributor.nonIdAuthor | Sun, Yujin | - |
dc.contributor.nonIdAuthor | Kim, Hoe Suk | - |
dc.contributor.nonIdAuthor | Park, Jinho | - |
dc.contributor.nonIdAuthor | Li, Mulan | - |
dc.contributor.nonIdAuthor | Tian, Lianji | - |
dc.contributor.nonIdAuthor | Choi, YoonSeok | - |
dc.contributor.nonIdAuthor | Choi, Byung Ihn | - |
dc.contributor.nonIdAuthor | Moon, Woo Kyung | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Breast tumor initiating cells | - |
dc.subject.keywordAuthor | Extra domain-B of fibronectin | - |
dc.subject.keywordAuthor | Aptides | - |
dc.subject.keywordAuthor | Superparamagnetic iron oxide nanoparticles | - |
dc.subject.keywordAuthor | Magnetic resonance imaging | - |
dc.subject.keywordPlus | SUPERPARAMAGNETIC IRON-OXIDE | - |
dc.subject.keywordPlus | ORAL SQUAMOUS-CELL | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MARKER | - |
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