Development of recombinant Chinese hamster ovary cell lines for therapeutic protein production

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The current biopharmaceutical market is driven by the steady increase in demand for recombinant therapeutics produced in mammalian cell lines. Chinese hamster ovary (CHO) cells are the predominant workhorses for large-scale stable expression of human glycoproteins. However, the low throughput and time-consuming process of cell line development, heterogeneity and instability of CHO cell lines pose serious challenges. This review highlights the conventional expression system with its limitations and the recent advances in high throughput screening methods for rapid clonal selection, targeted gene integration methods for precise prediction of transcriptional activities, and engineering of DNA elements to improve the stability of CHO cell lines.
Publisher
Elsevier BV
Issue Date
2013-11
Language
English
Article Type
Article
Citation

Current Opinion in Chemical Engineering, v.2, no.4, pp.391 - 397

ISSN
2211-3398
DOI
10.1016/j.coche.2013.08.002
URI
http://hdl.handle.net/10203/194171
Appears in Collection
BS-Journal Papers(저널논문)
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