A high-affinity peptide for nicotinic acetylcholine receptor-alpha 1 and its potential use in pulmonary drug delivery

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dc.contributor.authorPark, Sehoko
dc.contributor.authorKim, Young-Junko
dc.contributor.authorJon, Sangyongko
dc.date.accessioned2014-12-16T01:36:51Z-
dc.date.available2014-12-16T01:36:51Z-
dc.date.created2014-11-04-
dc.date.created2014-11-04-
dc.date.issued2014-10-
dc.identifier.citationJOURNAL OF CONTROLLED RELEASE, v.192, pp.141 - 147-
dc.identifier.issn0168-3659-
dc.identifier.urihttp://hdl.handle.net/10203/192931-
dc.description.abstractIn pulmonary drug delivery, the ability of an affinity molecule to bind to lung epithelium may prolong retention of therapeutic molecules within the lung and consequently yield higher overall bioavailability. To this end, we screened a library of structurally constrained peptides ('aptides') using phage-display technology and identified a high-affinity aptide for the mouse nicotinic acetylcholine receptor-alpha 1 (nAChR-alpha 1). The isolated aptide (APT(nAChR-alpha 1)) bound to its target protein with high affinity (K-d = 47 nM). Alexa 488-labeled APT(nAChR-alpha 1) showed preferential binding to nAChR-alpha 1-positive mouse lung epithelial cells and mouse muscle cells. Furthermore, the aptide exhibited substantial binding in nAChR-a1-positive tissue sections of muscle, trachea and lung, but not in liver, kidney or spleen tissues, which are nAChR-alpha 1-negative. In an in vivo experiment, a high-intensity fluorescence signal was observed in the entire lung up to 50 h after tracheal injection of Cy5.5-APTnAChR-alpha 1, whereas most of the fluorescence signal from a Cy5.5-labeled scrambled peptide washed out within 20 h after injection. Taken together, these results indicate that the high-affinity peptide for nAChR-alpha 1 identified here bound tightly to lung epithelium and thus exhibited a long residence time in this tissue, suggesting that the peptide could be used for pulmonary delivery of active pharmaceuticals.-
dc.languageEnglish-
dc.publisherELSEVIER SCIENCE BV-
dc.subjectMEDIATED TRANSCYTOSIS-
dc.subjectCYSTIC-FIBROSIS-
dc.subjectINHALATION-
dc.subjectINSULIN-
dc.subjectFIBRONECTIN-
dc.subjectABSORPTION-
dc.subjectEXPRESSION-
dc.subjectDEPOSITION-
dc.subjectPROTEINS-
dc.subjectHUMANS-
dc.titleA high-affinity peptide for nicotinic acetylcholine receptor-alpha 1 and its potential use in pulmonary drug delivery-
dc.typeArticle-
dc.identifier.wosid000342460400015-
dc.identifier.scopusid2-s2.0-84905166503-
dc.type.rimsART-
dc.citation.volume192-
dc.citation.beginningpage141-
dc.citation.endingpage147-
dc.citation.publicationnameJOURNAL OF CONTROLLED RELEASE-
dc.identifier.doi10.1016/j.jconrel.2014.07.006-
dc.contributor.localauthorJon, Sangyong-
dc.contributor.nonIdAuthorPark, Seho-
dc.contributor.nonIdAuthorKim, Young-Jun-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorAptides-
dc.subject.keywordAuthorNicotinic acetylcholine receptors-
dc.subject.keywordAuthorPulmonary delivery-
dc.subject.keywordAuthorPhage display-
dc.subject.keywordPlusMEDIATED TRANSCYTOSIS-
dc.subject.keywordPlusCYSTIC-FIBROSIS-
dc.subject.keywordPlusINHALATION-
dc.subject.keywordPlusINSULIN-
dc.subject.keywordPlusFIBRONECTIN-
dc.subject.keywordPlusABSORPTION-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusDEPOSITION-
dc.subject.keywordPlusPROTEINS-
dc.subject.keywordPlusHUMANS-
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