Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling

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Receptor tyrosine kinases (RTKs) are a family of cell-surface receptors that have a key role in regulating critical cellular processes. Here, to understand and precisely control RTK signalling, we report the development of a genetically encoded, photoactivatable Trk (tropomyosin-related kinase) family of RTKs using a light-responsive module based on Arabidopsis thaliana cryptochrome 2. Blue-light stimulation (488 nm) of mammalian cells harbouring these receptors robustly upregulates canonical Trk signalling. A single light stimulus triggers transient signalling activation, which is reversibly tuned by repetitive delivery of blue-light pulses. In addition, the light-provoked process is induced in a spatially restricted and cell-specific manner. A prolonged patterned illumination causes sustained activation of extracellular signal-regulated kinase and promotes neurite outgrowth in a neuronal cell line, and induces filopodia formation in rat hippocampal neurons. These light-controllable receptors are expected to create experimental opportunities to spatiotemporally manipulate many biological processes both in vitro and in vivo.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2014-06
Language
English
Article Type
Article
Keywords

NERVE GROWTH-FACTOR; PROTEIN INTERACTIONS; TRK RECEPTORS; ACTIVATION; INDUCTION; CELLS; TRANSDUCTION; DIFFERENTIATION; PARTNERS; LIGAND

Citation

NATURE COMMUNICATIONS, v.5, pp.4057

ISSN
2041-1723
DOI
10.1038/ncomms5057
URI
http://hdl.handle.net/10203/189866
Appears in Collection
BS-Journal Papers(저널논문)
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