2,3-Butanediol recovery from fermentation broth by alcohol precipitation and vacuum distillation

Cited 15 time in webofscience Cited 0 time in scopus
  • Hit : 333
  • Download : 0
This study presents a new and effective downstream process to recover 2,3-butanediol (2,3-BD) from fermentation broth which is produced by a recombinant Klaebsiella pneumoniae strain. The IdhA-deficient K. pneumoniae strain yielded about 90 g/L of 2,3-BD, along with a number of by-products, such as organic acids and alcohols, in a 65 h fed-batch fermentation. The pH-adjusted cell-free fermentation broth was firstly concentrated until 2,3-BD reached around 500 g/L by vacuum evaporation at 50 degrees C and 50 mbar vacuum pressure. The concentrated solution was further treated using light alcohols, including methanol, ethanol, and isopropanol, for the precipitation of organic acids and inorganic salts. Isopropanol showed the highest removal efficiency, in which 92.5% and 99.8% of organic acids and inorganic salts were precipitated, respectively. At a final step, a vacuum distillation process enabled the recovery of 76.2% of the treated 2,3-BD, with 96.1% purity, indicating that fermentatively produced 2,3-BD is effectively recovered by a simple alcohol precipitation and vacuum distillation.
Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
Issue Date
2014-04
Language
English
Article Type
Article
Keywords

1,3-PROPANEDIOL FERMENTATION; KLEBSIELLA-PNEUMONIAE; ACETIC-ACID; GLYCEROL; ELECTRODIALYSIS; DEHYDROGENASE; DESALINATION; EXTRACTION; OXYTOCA; STATE

Citation

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v.117, no.4, pp.464 - 470

ISSN
1389-1723
DOI
10.1016/j.jbiosc.2013.09.007
URI
http://hdl.handle.net/10203/189312
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 15 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0