Bioluminescence induced reactive oxygen species generation for photodynamic therapy = 광역동 치료에 이용하기 위한 생체 발광으로 유도된 활성산소의 생성

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Photodynamic therapy (PDT) has developed as one of promising systems in cancer therapy for its high-selectivity and low systemic cytotoxicity. However, the accessibility of external light limits the application on deeper tumor because of tissue-penetrating properties. In this study, bioluminescence (BL) enzyme protein- photosensitizer (PS) conjugates were synthesized using covalent bonging. Renilla luciferase8 (Rluc8), mutant of enzyme protein that has stability and high intensity BL, was employed as an internal light source. Chlorin e6, photosensitizer that has high singlet oxygen quantum yield, was covalently attached to Rluc8. Generation of reactive oxygen species (ROS) could be achieved through bioluminescence resonance energy transfer (BRET) from BL to photosensitizer. The amount of ROS produced from Rluc8-e6 conjugates was detected using singlet oxygen sensor green (SOSG), which shows fluorescence increase as ROS is generated. Our results show that Rluc8-e6 conjugates could be synthesized without distorting of properties of both materials and conjugates could generate ROS, which may be applied for PDT.
Advisors
Yun, Seok-Hyun윤석현
Description
한국과학기술원 : 나노과학기술학과,
Publisher
한국과학기술원
Issue Date
2013
Identifier
514918/325007  / 020118095
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 나노과학기술학과, 2013.2, [ iv, 43 p. ]

Keywords

bioluminescence resonance energy transfer; photosensitizer; reactive oxygen species; 생체 발광 단백질; 광감음제; 광역동 치료; 활성산소; 생체 발광 성 공명 에너지 전이; photodynamic therapy

URI
http://hdl.handle.net/10203/182267
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=514918&flag=dissertation
Appears in Collection
NT-Theses_Master(석사논문)
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