Economical viability and natural potential as a carbon source have motivated engineering Mannheimia succiniciproducens for the production of succinic acid through glycerol fermentation1. The methods of rational approaches and adaptive evolution were used to improve the ability of utilization of glycerol as a carbon source in M. succinicproducens. The performance of engineered strains such as PALK-glpA, PALK-glpAcrp, PALK -glpFKA, and PALK-sldABdhaK was better than that of control strain in batch fermentation experiments Above all, in fed-batch fermentation, the most productive strain harboring C.acetobutylicum ATCC824 glycerol 3-phosphate dehydrogenase, and M.succiniciproducens cAMP receptor protein increased both concentration and productivity of succinic acid.Surprisingly, expression of the genes encoding the glycerol dehydrogenase and dihydroxyacetone kinase from G.oxydans was able to improve the rate of glycerol utilization and the production of succinic acid significantly as previously reported in E.coli.