A semi-automated vibriocidal assay for improved measurement of cholera vaccine-induced immune responses

Vibriocidal antibody assay has been a surrogate standard assay in the evaluation of cholera vaccine efficacy because it has a good correlation with protection. Although the optical density-based vibriocidal assay in a 96-well microtiter-plate format is widely used in clinical trials, it has limitations as vibriocidal titers are altered by incubation time and samples with the same end-point titers could have potentially different vibriocidal kinetics. In the present study, we developed an improved agar-plate assay coupled with an automated colony counting system. Through testing 30 pairs of human sera from vaccinces administered with a cholera vaccine or placebo, these two assays showed good correlations for the vibriocidal titers and fold increases in titers between pre- and post-vaccinated sera as determined by the Pearson correlation coefficient and the Regression coefficient. Notably, the newly-developed semi-automated assay demonstrated that serum samples with the same end-point titers turned out to have distinct vibriocidal kinetics that were not distinguishable by the microtiter-plate assay. The semi-automated assay responded, p specifically to vibrio cholerae but not to irrelevant bacteria such as Salmonella typhi and Escherichia coli. These results demonstrate that the semi-automated assay provides better sensitivity, accuracy, and stability of the assay results with minimized efforts than conventional microtiterplate assay and could provide a useful tool as an in vitro surrogate assay for the evaluation of cholera vaccine efficacy. (c) 2007 Elsevier B.V. All rights reserved.
Publisher
Elsevier Science Bv
Issue Date
2007-11
Language
ENG
Keywords

WHOLE-CELL; ANTIBODIES; TRIAL

Citation

JOURNAL OF MICROBIOLOGICAL METHODS, v.71, no.2, pp.141 - 146

ISSN
0167-7012
DOI
10.1016/j.mimet.2007.08.009
URI
http://hdl.handle.net/10203/14104
Appears in Collection
BS-Journal Papers(저널논문)
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