Highly open porous biodegradable microcarriers: In vitro cultivation of chondrocytes for injectable delivery

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Injectable cell therapy would provide a patient-friendly procedure for treatment of degenerated or wounded tissue. Biodegradable injectable porous microspheres were fabricated to use as dual-purpose microcarriers for cell culture and injectable scaffold for tissue regeneration. Gas foaming in a water-in-oil-in-water double emulsion was performed for fabricating the well-interconnected porous microcarriers using poly(lactic-co-glycolic acid) (PLGA). The gas foaming conditions were finely tuned to control the structural and morphological characteristics. Porous microcarriers with a mean size of approximately 175 mu m and an average pore diameter of approximately 29 mu m were produced for cell cultivation and injectable delivery. To promote cell seeding, amine-functionalized porous microcarriers were prepared by blending amine-functionalized PLGA with unreacted PLGA. To assess the porous microcarriers for chondrocyte cultivation, bovine articular chondrocytes were seeded and cultured in vitro in spinner flasks for 4 weeks. Visualization and biochemical analyses of the microcarrier-cell constructs were performed to demonstrate cell proliferation and phenotypic expression. Quantification of deoxyribonucleic acid, glycosaminoglycan, and collagen content showed that much greater cell proliferation and expression of cartilage-specific phenotype were observed for cultures in the following order: amine-functionalized porous microcarriers, porous microcarriers, nonporous microcarriers, and monolayer culture.
Publisher
MARY ANN LIEBERT INC
Issue Date
2008-05
Language
English
Article Type
Article
Keywords

CELL ATTACHMENT; POLYMERIC MICROSPHERES; ROTATING BIOREACTOR; SCAFFOLDS; CULTURE; DESIGN; GROWTH; EXPANSION; SYSTEM; ACID)

Citation

TISSUE ENGINEERING, v.14, no.5, pp.607 - 615

ISSN
2152-4947
DOI
10.1089/tea.2007.0263
URI
http://hdl.handle.net/10203/13131
Appears in Collection
NT-Journal Papers(저널논문)BS-Journal Papers(저널논문)
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