G-CSF loaded biodegradable PLGA nanoparticles prepared by a single oil-in-water emulsion method

Cited 42 time in webofscience Cited 0 time in scopus
  • Hit : 449
  • Download : 241
A new formulation method was developed for preparing poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles loaded with recombinant human granulocyte colony-stimulating factor (rhG-CSF). Lyophilized rhG-CSF powder and PLGA polymer were directly co-dissolved in a single organic phase, and the resulting solution was dispersed into an aqueous Solution. PLGA nanoparticles encapsulating rhG-CSF were produced by a spontaneous emulsion/solvent diffusion method. In this manner, rhG-CSF was molecularly dissolved in the polymer phase. Release profile of rhG-CSF from PLGA nanoparticles was compared with those from two kinds of PLGA microparticles which were separately prepared by either single oil-in-water (O/W) or double water-in-oil-in-water (W/O/W) emulsion technique. The sizes of rhG-CSF loaded nanoparticles, O/W microparticles, and W/O/W microparticles were about 257 nm, 4.7 mu m, and 4.3 mu m, respectively. For rhG-CSF nanoparticles,about 90% of encapsulated rhG-CSF was released out in a sustained manner from PLGA nanoparticles over a 1 week period, but for rhG-CSF microparticles, only about 20% of rhG-CSF Could be released out during the same period. Reversed phase and size exclusion chromatograms revealed that the structural integrity of released rhG-CSF from nanoparticles was nearly intact, compared to that of native rhG-CSF. (c) 2005 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2006-03
Language
English
Article Type
Article
Keywords

POLY(D,L-LACTIC-CO-GLYCOLIC ACID) MICROSPHERES; COLONY-STIMULATING FACTOR; HUMAN GROWTH-HORMONE; CONTROLLED DELIVERY; RELEASE MECHANISM; ORGANIC-SOLVENTS; PROTEIN RELEASE; STABILITY; MICROPARTICLES; MICROENCAPSULATION

Citation

INTERNATIONAL JOURNAL OF PHARMACEUTICS, v.311, no.1-2, pp.223 - 228

ISSN
0378-5173
DOI
10.1016/j.ijpharm.2005.12.023
URI
http://hdl.handle.net/10203/12339
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 42 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0