Surface-initiated growth of poly d(A-T) by Taq DNA polymerase

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In this paper, we report surface-initiated d(A-T) polymerization by Taq DNA polymerase as a method for constructing DNA-tethered surfaces using an enzyme. The enzymatic polymerization was conducted successfully via two steps: tethering of oligo d(A-T)s onto the surface presenting carboxylic acids by amide coupling and surface-initiated polymerization using Taq DNA polymerase. In this enzymatic polymerization process, the design and construction of carboxylic acid-presenting surfaces were found to be an important factor: DNA growth did not occur on the gold surface coated only with the self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA), but effectively proceeded on the surfaces presenting mixed SAMs of MHDA and 1-pentadecanethiol. The coupling of oligo d(A-T)s and the subsequent DNA polymerization reaction were characterized by polarized infrared external reflectance spectroscopy, ellipsometry, X-ray photoelectron spectroscopy, and atomic force microscopy.
Publisher
AMER CHEMICAL SOC
Issue Date
2005-05
Language
English
Article Type
Article
Keywords

SELF-ASSEMBLED MONOLAYERS; GOLD SURFACES; OLIGONUCLEOTIDE ARRAYS; PLASMON RESONANCE; STRANDED-DNA; IMMOBILIZATION; HYBRIDIZATION; POLYMERIZATION; MICROARRAYS; ATTACHMENT

Citation

LANGMUIR, v.21, no.10, pp.4669 - 4673

ISSN
0743-7463
DOI
10.1021/la046819y
URI
http://hdl.handle.net/10203/12155
Appears in Collection
CH-Journal Papers(저널논문)
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