Streptomyces panacagri sp nov., isolated from soil of a ginseng field

Abstract

A Gram-positive, spore-forming, aerobic actinomycete, strain Gsoil 519(T), was isolated from soil of a ginseng field of Pocheon province in South Korea. The closest phylogenetic relatives were Streptomyces marinus Sp080513GE-26(T) (97.94% 16S rRNA gene sequence similarity), Streptomyces albiaxialis NRRL B-24327(T) (97.84%), Streptomyces albus subsp. albus DSM 40313(T) (97.84%), Streptomyces almquistii NBRC 13015(T) (97.81 %), Streptomyces gibsonii NBRC 15415(T) (97.81%), Streptomyces rangoonensis NBRC 13078(T) (97.81%), Streptomyces sodiiphilus YIM 80305(T) (97.77%) and Streptomyces flocculus NBRC 13041(T) (97.67 %). The G+C content of the genomic DNA was 71.8 molo%. The chemotaxonomic data [MK-9(H-6) and MK-9(H-8) as the major menaquinones; LL-diaminopimelic acid as a component of the cell-wall peptidoglycan; ribose, xylose, mannose and glucose as the major cell-wall sugars; and anteiso-C-15:0 iso-C-15:0 iSO-C-17:0 anteiso-C-17:0 and C-16:0 as the major fatty acids] supported the affiliation of strain Gsoil 519(T) to the genus Streptomyces. The physiological and biochemical characteristics and the low level of DNA DNA relatedness differentiated the isolate genotypically and phenotypically from recognized members of the genus Streptomyces. The isolate, therefore, represents a novel species, for which the name Streptomyces panacagri sp. nov. is proposed, with Gsoil 519(T) (=KCTC 19139(T)=DSM 41871(T)) as the type strain.