Current state and perspectives on erythropoietin production

Cited 17 time in webofscience Cited 0 time in scopus
  • Hit : 249
  • Download : 0
Erythropoietin is a major regulator of erythropoiesis which maintains the body's red blood cell mass and tissue oxygenation at an optimum level. Recombinant human erythropoietin (rhEPO), which is a widely used therapeutic agent for the treatment of anemia and which represents one of the largest biopharmaceuticals markets, is produced from recombinant Chinese hamster ovary cells. rhEPO is a glycoprotein with complex glycan structure, which is responsible for its therapeutic efficacy, including the in vivo activity and half-life. In order to obtain an optimal and consistent glycoform profile of rhEPO and concurrently maintain a high production yield, various approaches in drug development and cell culture technology have been attempted. Recent advances in rhEPO production are classified into three types: the development of improved rhEPO molecules by protein engineering; improvement of production host cells by genetic engineering; and culture condition optimization by fine control of the production mode/system, process parameters, and culture media. In this review, we focus on rhEPO production strategies as they have progressed thus far. Furthermore, the current status of the market and outlook on rhEPO and its derivatives are discussed.
Publisher
SPRINGER
Issue Date
2012-09
Language
English
Article Type
Review
Keywords

HAMSTER OVARY CELLS; RECOMBINANT-HUMAN-ERYTHROPOIETIN; N-GLYCOLYLNEURAMINIC ACID; EXPRESSING CHO-CELLS; ENHANCED SIALYLATION; CULTURE-CONDITIONS; SIALIC-ACID; SODIUM-BUTYRATE; PICHIA-PASTORIS; GLYCOSYLATION

Citation

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.95, no.6, pp.1405 - 1416

ISSN
0175-7598
DOI
10.1007/s00253-012-4291-x
URI
http://hdl.handle.net/10203/104079
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 17 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0