A Novel Molecular Beacon Bearing a Graphite Nanoparticle as a Nanoquencher for In situ mRNA Detection in Cancer Cells

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Molecular beacons (MBs) have shown fascinating applications in many biological fields. However, exploration of cost-effective, sensitive, stable and efficient MB for in situ live cell- based assay has still room for improvement. In this regards, we have developed a novel MB which bears a spherical graphite nanoparticle (GN) as a fluorescent quencher. The GN resulted in the high quenching efficiency, and the presence of GN enhanced the biological stability and transfection of the MB into the cells, thereby enabling the real-time survivin mRNA detection and quantification in the MCF-7 breast cancer cells. These results demonstrated that the advancement of the proposed MB containing a GN nanoquencher can be used as a robust molecular probe for genetic analysis in the cells.
Publisher
AMER CHEMICAL SOC
Issue Date
2012-12
Language
English
Article Type
Article
Keywords

RESONANCE ENERGY-TRANSFER; QUENCHED FLUORESCENT OLIGONUCLEOTIDES; GRAPHENE OXIDE; LIVING CELLS; HYBRIDIZATION; PROBES; PCR

Citation

ACS APPLIED MATERIALS & INTERFACES, v.4, no.12, pp.6784 - 6788

ISSN
1944-8244
DOI
10.1021/am301976r
URI
http://hdl.handle.net/10203/102353
Appears in Collection
CBE-Journal Papers(저널논문)
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