A simple gold nanoparticle-mediated immobilization method to fabricate highly homogeneous DNA microarrays having higher capacities than those prepared by using conventional techniques

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A simple, highly efficient immobilization method to fabricate DNA microarrays, that utilizes gold nanoparticles as the mediator, has been developed. The fabrication method begins with electrostatic attachment of amine-modified DNA to gold nanoparticles. The resulting gold-DNA complexes are immobilized on conventional amine or aldehyde functionalized glass slides. By employing gold nanoparticles as the immobilization mediator, implementation of this procedure yields highly homogeneous microarrays that have higher binding capacities than those produced by conventional methods. This outcome is due to the increased three-dimensional immobilization surface provided by the gold nanoparticles as well as the intrinsic effects of gold on emission properties. This novel immobilization strategy gives microarrays that produce more intense hybridization signals for the complementary DNA. Furthermore, the silver enhancement technique, made possible only in the case of immobilized gold nanoparticles on the microarrays, enables simple monitoring of the integrity of the immobilized DNA probe.
Publisher
IOP PUBLISHING LTD
Issue Date
2009-01
Language
English
Article Type
Article
Keywords

OLIGONUCLEOTIDE MICROCHIPS; COLORIMETRIC DETECTION; HNF-1-ALPHA MUTATIONS; GENOMIC DNA; FLUORESCENCE; PROBES; HYBRIDIZATION; SEQUENCES; DIAGNOSIS; SURFACE

Citation

NANOTECHNOLOGY, v.20, no.3

ISSN
0957-4484
DOI
10.1088/0957-4484/20/3/035607
URI
http://hdl.handle.net/10203/101202
Appears in Collection
CBE-Journal Papers(저널논문)
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