Enhanced Interferon-beta Production by CHO Cells Through Elevated Osmolality and Reduced Culture Temperature

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dc.contributor.authorHan, Young-Kueko
dc.contributor.authorKoo, Tai-Youngko
dc.contributor.authorLee, Gyun-Minko
dc.date.accessioned2013-03-12T01:34:22Z-
dc.date.available2013-03-12T01:34:22Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2009-09-
dc.identifier.citationBIOTECHNOLOGY PROGRESS, v.25, no.5, pp.1440 - 1447-
dc.identifier.issn8756-7938-
dc.identifier.urihttp://hdl.handle.net/10203/100974-
dc.description.abstractFor efficient production of native interferon-beta (IFN-beta) in recombinant CHO cell culture, the IFN-beta molecular aggregation that occurs during culture needs to be minimized. To do so, we investigated the effect of hyperosmolality and hypothermia on IFN-beta production and molecular aggregation in rCHO cell culture. Both hyperosmolality (470 mOsm/kg) and hypothermia (32 degrees C) increased specific native INF-beta productivity q(IFN-beta). Furthermore, they decreased the IFN-beta molecular aggregation, although severe IFN-beta molecular aggregation could not be avoided in the later phase of culture. To overcome growth suppression at hyperosmolality and hypothermia, cells were cultivated in a biphasic mode. Cells were first cultivated at 310 mOsm/kg and 37 degrees C for 2 days to rapidly obtain a reasonably high cell concentration. The, temperature and osmolality were then shifted to 32 degrees C and 470 mOsm/kg, respectively, to achieve high q(IFN-beta) and reduced IFN-beta molecular aggregation. Due to the enhanced q(IFN-beta) and delayed molecular aggregation, the highest native IFN-beta concentration achieved on day 6 was 18.03 +/- 0.61 mg/L, which was 5.30-fold higher than that in a control batch culture (310 mOsm/kg and 37 degrees C). Taken together, a combination of hyperosmolality and hypothermia in a biphasic culture is a useful strategy for improved native IFN-beta production from rCHO cells. (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 25: 1440-1447, 2009-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.subjectHAMSTER OVARY CELLS-
dc.subjectHUMAN FIBROBLAST INTERFERON-
dc.subjectKERATINOCYTE GROWTH-FACTOR-
dc.subjectPH-
dc.subjectERYTHROPOIETIN-
dc.subjectSTABILIZATION-
dc.subjectMECHANISMS-
dc.subjectEXPRESSION-
dc.subjectSUSPENSION-
dc.subjectREDUCTION-
dc.titleEnhanced Interferon-beta Production by CHO Cells Through Elevated Osmolality and Reduced Culture Temperature-
dc.typeArticle-
dc.identifier.wosid000271950200028-
dc.identifier.scopusid2-s2.0-70350141643-
dc.type.rimsART-
dc.citation.volume25-
dc.citation.issue5-
dc.citation.beginningpage1440-
dc.citation.endingpage1447-
dc.citation.publicationnameBIOTECHNOLOGY PROGRESS-
dc.identifier.doi10.1002/btpr.234-
dc.contributor.localauthorLee, Gyun-Min-
dc.contributor.nonIdAuthorKoo, Tai-Young-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorCHO cells-
dc.subject.keywordAuthorhyperosmolality-
dc.subject.keywordAuthorhypothermia-
dc.subject.keywordAuthorinterferon-beta-
dc.subject.keywordAuthormolecular aggregation-
dc.subject.keywordPlusHAMSTER OVARY CELLS-
dc.subject.keywordPlusHUMAN FIBROBLAST INTERFERON-
dc.subject.keywordPlusKERATINOCYTE GROWTH-FACTOR-
dc.subject.keywordPlusPH-
dc.subject.keywordPlusERYTHROPOIETIN-
dc.subject.keywordPlusSTABILIZATION-
dc.subject.keywordPlusMECHANISMS-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusSUSPENSION-
dc.subject.keywordPlusREDUCTION-
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