DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Yeon-Gu | ko |
dc.contributor.author | Kim, Jee Yon | ko |
dc.contributor.author | Park, Byoungwoo | ko |
dc.contributor.author | Ahn, Jung Oh | ko |
dc.contributor.author | Jung, Joon-Ki | ko |
dc.contributor.author | Lee, Hong Weon | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.contributor.author | Lee, Eun Gyo | ko |
dc.date.accessioned | 2013-03-12T00:58:34Z | - |
dc.date.available | 2013-03-12T00:58:34Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-11 | - |
dc.identifier.citation | PROCESS BIOCHEMISTRY, v.46, no.11, pp.2201 - 2204 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | http://hdl.handle.net/10203/100891 | - |
dc.description.abstract | Dimethyl sulfoxide (DMSO) can increase the specific productivity (q) of foreign proteins in mammalian cells, while it can also induce cell death, particularly apoptosis. Bcl-xL is a typical anti-apoptotic protein that inhibits the apoptosis in recombinant Chinese hamster ovary (rCHO) cell culture. To evaluate the potential role of Bcl-xL overexpression on DMSO-mediated erythropoietin (EPO) production, we used EPO-producing rCHO cells with regulated Bcl-xL overexpression (EPO-off-Bcl-xL) by doxycycline. Although DMSO addition enhanced specific EPO productivity (q(Epo)), it also induced cell death in EPO-off-Bcl-xL cells. Bcl-xL overexpression reduced the DMSO-induced cell death followed by release of various enzymes from plasma membrane-damaged cells as evidenced from LDH assay, resulting in delayed loss of EPO. However, it did not significantly improve the maximum EPO production. In addition, Bcl-xL overexpression suppressed DMSO-induced apoptosis, characterized by DNA fragmentation and Annexin V staining. Taken together, Bcl-xL overexpression could inhibit DMSO-induced apoptosis, thereby delaying the loss of EPO. (C) 2011 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | SURFACE-ANTIGEN EXPRESSION | - |
dc.subject | ANTITHROMBIN-III | - |
dc.subject | BATCH CULTURE | - |
dc.subject | CHO-CELLS | - |
dc.subject | APOPTOSIS | - |
dc.subject | IMPROVEMENT | - |
dc.subject | ACTIVATION | - |
dc.subject | KINETICS | - |
dc.subject | ANTIBODY | - |
dc.subject | SYSTEMS | - |
dc.title | Effect of Bcl-xL overexpression on erythropoietin production in recombinant Chinese hamster ovary cells treated with dimethyl sulfoxide | - |
dc.type | Article | - |
dc.identifier.wosid | 000297237400015 | - |
dc.identifier.scopusid | 2-s2.0-80053978385 | - |
dc.type.rims | ART | - |
dc.citation.volume | 46 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 2201 | - |
dc.citation.endingpage | 2204 | - |
dc.citation.publicationname | PROCESS BIOCHEMISTRY | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Kim, Yeon-Gu | - |
dc.contributor.nonIdAuthor | Kim, Jee Yon | - |
dc.contributor.nonIdAuthor | Park, Byoungwoo | - |
dc.contributor.nonIdAuthor | Ahn, Jung Oh | - |
dc.contributor.nonIdAuthor | Jung, Joon-Ki | - |
dc.contributor.nonIdAuthor | Lee, Hong Weon | - |
dc.contributor.nonIdAuthor | Lee, Eun Gyo | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Dimethyl sulfoxide | - |
dc.subject.keywordAuthor | Bcl-xL | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | rCHO cells | - |
dc.subject.keywordAuthor | Inducible expression | - |
dc.subject.keywordPlus | SURFACE-ANTIGEN EXPRESSION | - |
dc.subject.keywordPlus | ANTITHROMBIN-III | - |
dc.subject.keywordPlus | BATCH CULTURE | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | IMPROVEMENT | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | KINETICS | - |
dc.subject.keywordPlus | ANTIBODY | - |
dc.subject.keywordPlus | SYSTEMS | - |
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