Isolation and characterization of algicidal bacteria from Cochlodinium polykrikoides culture

Cited 22 time in webofscience Cited 0 time in scopus
  • Hit : 371
  • Download : 0
In this study, we analyzed a bacterial community closely associated with Cochlodinium polykrikoides that caused harmful algal blooming in the sea. Filtration using a plankton mesh and percoll gradient centrifugation were performed to eliminate free-living bacteria. Attached bacteria were analyzed by culture-dependent and culture-independent methods. Five culturable bacterial strains were isolated and identified from the C. polykrikoides mixed bacterial community. The isolates belonged to alpha-Proteobacteria (Nautella sp., Sagittula sp., and Thalassobius sp.) and gamma-Proteobacteria (Alteromonas sp. and Pseudoalteromonas sp.). All of the 5 isolates showed algicidal activity against C. polykrikoides and produced extracellular compounds responsible for algicidal properties after entering the stationary phase. The algicidal compounds produced by the 5 isolates were heat-stable and had molecular masses of less than 10,000 Da. Furthermore, the algicidal compounds were relatively specific for C. polykrikoides in terms of their algicidal activities. Culture-independent analysis of the bacterial community in association with C. polykrikoides was performed using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). On the basis of the PCR-DGGE profile, Sagittula sp. was identified as a dominant species in the bacterial community of C. polykrikoides.
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Issue Date
2011
Language
English
Article Type
Article
Keywords

MARINE BACTERIUM; CLASS PROTEOBACTERIA; COMMUNITY DYNAMICS; ORGANIC-MATTER; ALGAL BLOOMS; DINOFLAGELLATE; PHYTOPLANKTON; GYMNODINIUM; SEA; RED

Citation

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.16, no.6, pp.1124 - 1133

ISSN
1226-8372
URI
http://hdl.handle.net/10203/99921
Appears in Collection
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 22 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0