DC Field | Value | Language |
---|---|---|
dc.contributor.author | Um, Eu-Jin | ko |
dc.contributor.author | Lee, Seung-Goo | ko |
dc.contributor.author | Park, Je-Kyun | ko |
dc.date.accessioned | 2013-03-11T17:46:35Z | - |
dc.date.available | 2013-03-11T17:46:35Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-10 | - |
dc.identifier.citation | APPLIED PHYSICS LETTERS, v.97, no.15, pp.153703 | - |
dc.identifier.issn | 0003-6951 | - |
dc.identifier.uri | http://hdl.handle.net/10203/99772 | - |
dc.description.abstract | Microfluidic droplet-based technology enables encapsulation of cells in the isolated aqueous chambers surrounded by immiscible fluid but single-cell encapsulation efficiency is usually less than 30%. In this letter, we introduce a simple microgroove structure to break droplets into random sizes which further allows collecting of single-cell [Escherichia coli ( E. coli)] containing droplets by their size differences. Pinched-flow separation method is integrated to sort out droplets of certain sizes which have high probability of containing one cell. Consequently, we were able to obtain more than 50% of droplets having single E. coli inside, keeping the proportion of multiple-cell containing droplets less than 16%. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3500980] | - |
dc.language | English | - |
dc.publisher | AMER INST PHYSICS | - |
dc.subject | DROPLETS | - |
dc.subject | MICROFLUIDICS | - |
dc.subject | MICROCHANNELS | - |
dc.title | Random breakup of microdroplets for single-cell encapsulation | - |
dc.type | Article | - |
dc.identifier.wosid | 000283216900092 | - |
dc.identifier.scopusid | 2-s2.0-77958107883 | - |
dc.type.rims | ART | - |
dc.citation.volume | 97 | - |
dc.citation.issue | 15 | - |
dc.citation.beginningpage | 153703 | - |
dc.citation.publicationname | APPLIED PHYSICS LETTERS | - |
dc.identifier.doi | 10.1063/1.3500980 | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.contributor.nonIdAuthor | Lee, Seung-Goo | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | DROPLETS | - |
dc.subject.keywordPlus | MICROFLUIDICS | - |
dc.subject.keywordPlus | MICROCHANNELS | - |
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