RBSDesigner: software for designing synthetic ribosome binding sites that yields a desired level of protein expression

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Motivation: RBSDesigner predicts the translation efficiency of existing mRNA sequences and designs synthetic ribosome binding sites (RBSs) for a given coding sequence (CDS) to yield a desired level of protein expression. The program implements the mathematical model for translation initiation described in Na et al. (Mathematical modeling of translation initiation for the estimation of its efficiency to computationally design mRNA sequences with a desired expression level in prokaryotes. BMC Syst. Biol., 4, 71). The program additionally incorporates the effect on translation efficiency of the spacer length between a Shine-Dalgarno (SD) sequence and an AUG codon, which is crucial for the incorporation of fMet-tRNA into the ribosome. RBSDesigner provides a graphical user interface (GUI) for the convenient design of synthetic RBSs.
Publisher
OXFORD UNIV PRESS
Issue Date
2010-10
Language
English
Article Type
Article
Keywords

TRANSLATION INITIATION; ESCHERICHIA-COLI; EFFICIENCY; SEQUENCES

Citation

BIOINFORMATICS, v.26, no.20, pp.2633 - 2634

ISSN
1367-4803
DOI
10.1093/bioinformatics/btq458
URI
http://hdl.handle.net/10203/99747
Appears in Collection
BiS-Journal Papers(저널논문)
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