Speckle-field digital holographic microscopy

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The use of coherent light in conventional holographic phase microscopy (HPM) poses three major drawbacks: poor spatial resolution, weak depth sectioning, and fixed pattern noise due to unwanted diffraction. Here, we report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging. A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability. (C) 2009 Optical Society of America
Publisher
OPTICAL SOC AMER
Issue Date
2009-07
Language
English
Article Type
Article
Citation

OPTICS EXPRESS, v.17, no.15, pp.12285 - 12292

ISSN
1094-4087
URI
http://hdl.handle.net/10203/99082
Appears in Collection
PH-Journal Papers(저널논문)
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