DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwak, Youn-Sig | ko |
dc.contributor.author | Han, Sangjo | ko |
dc.contributor.author | Thomashow, Linda S. | ko |
dc.contributor.author | Rice, Jennifer T. | ko |
dc.contributor.author | Paulitz, Timothy C. | ko |
dc.contributor.author | Kim, Dongsup | ko |
dc.contributor.author | Weller, David M. | ko |
dc.date.accessioned | 2013-03-11T09:19:43Z | - |
dc.date.available | 2013-03-11T09:19:43Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.77, no.5, pp.1770 - 1776 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/98891 | - |
dc.description.abstract | 2,4-Diacetylphloroglucinol (2,4-DAPG), an antibiotic produced by Pseudomonas fluorescens, has broad-spectrum antibiotic activity, inhibiting organisms ranging from viruses, bacteria, and fungi to higher plants and mammalian cells. The biosynthesis and regulation of 2,4-DAPG in P. fluorescens are well described, but the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a deletion library of Saccharomyces cerevisiae in broth and agar medium supplemented with 2,4-DAPG. We identified 231 mutants that showed increased sensitivity to 2,4-DAPG under both conditions, including 22 multidrug resistance-related mutants. Three major physiological functions correlated with an increase in sensitivity to 2,4-DAPG: membrane function, reactive oxygen regulation, and cell homeostasis. Physiological studies with wild-type yeast validated the results of the mutant screens. The chemical-genetic fitness profile of 2,4-DAPG resembled those of menthol, sodium azide, and hydrogen peroxide determined in previous high-throughput screening studies. Collectively, these findings indicate that 2,4-DAPG acts on multiple basic cellular processes. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | VACUOLAR H+-ATPASE | - |
dc.subject | RESISTANT STAPHYLOCOCCUS-AUREUS | - |
dc.subject | ALL DECLINE SOILS | - |
dc.subject | MULTIDRUG-RESISTANCE | - |
dc.subject | GENE ENCODES | - |
dc.subject | MARINE ALGA | - |
dc.subject | V-ATPASE | - |
dc.subject | SUBUNIT | - |
dc.subject | YEAST | - |
dc.subject | MECHANISMS | - |
dc.title | Saccharomyces cerevisiae Genome-Wide Mutant Screen for Sensitivity to 2,4-Diacetylphloroglucinol, an Antibiotic Produced by Pseudomonas fluorescens | - |
dc.type | Article | - |
dc.identifier.wosid | 000287700100030 | - |
dc.identifier.scopusid | 2-s2.0-79953165010 | - |
dc.type.rims | ART | - |
dc.citation.volume | 77 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 1770 | - |
dc.citation.endingpage | 1776 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.identifier.doi | 10.1128/AEM.02151-10 | - |
dc.contributor.localauthor | Kim, Dongsup | - |
dc.contributor.nonIdAuthor | Kwak, Youn-Sig | - |
dc.contributor.nonIdAuthor | Han, Sangjo | - |
dc.contributor.nonIdAuthor | Thomashow, Linda S. | - |
dc.contributor.nonIdAuthor | Rice, Jennifer T. | - |
dc.contributor.nonIdAuthor | Paulitz, Timothy C. | - |
dc.contributor.nonIdAuthor | Weller, David M. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | VACUOLAR H+-ATPASE | - |
dc.subject.keywordPlus | RESISTANT STAPHYLOCOCCUS-AUREUS | - |
dc.subject.keywordPlus | ALL DECLINE SOILS | - |
dc.subject.keywordPlus | MULTIDRUG-RESISTANCE | - |
dc.subject.keywordPlus | GENE ENCODES | - |
dc.subject.keywordPlus | MARINE ALGA | - |
dc.subject.keywordPlus | V-ATPASE | - |
dc.subject.keywordPlus | SUBUNIT | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | MECHANISMS | - |
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