Effects of Overexpression of C5 Protein on rnpB Gene Expression in Escherichia coli

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Escherichia coli RNase P is composed of a large RNA subunit (M I RNA) and a small protein subunit (C5 protein). Since both subunits are assembled in a 1: 1 ratio, expression of M I RNA and C5 protein should be coordinately regulated for RNase P to be efficiently synthesized in the cell. However, it is not known yet how the coordination occurs. In this study, we investigated how overexpression of C5 protein affects expression of the rnpB gene encoding M I RNA, using a lysogenic strain, which carries an rnpB-lacZ transcription fusion. Primer extension analysis of rnpB-lacZ fusion transcripts showed that the overexpression of C5 protein increased the amount of the fusion transcripts, suggesting that rnpB expression increases with the increase of intracellular level of C5 protein.
Publisher
KOREAN CHEMICAL SOC
Issue Date
2009-04
Language
English
Article Type
Article
Keywords

4.5 S-RNA; RIBONUCLEASE-P; NUCLEOTIDE-SEQUENCE; MESSENGER-RNAS; PRECURSOR; COMPONENT; MUTANTS; SUBSTRATE; CLEAVAGE; KINETICS

Citation

BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.30, no.4, pp.791 - 793

ISSN
0253-2964
URI
http://hdl.handle.net/10203/98799
Appears in Collection
CH-Journal Papers(저널논문)
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