Multipotent PDGFR beta-expressing cells in the circulation of stroke patients

Abstract

Tissue pericytes respond to injury, and support vascular and tissue regeneration. The presence of pericytes in the circulation may provide an attractive framework for tissue regeneration. Here, we detected multipotent pericyte-like cells in the circulating blood and determined its profiles during cerebral ischemia. Pericyte-like cells were isolated from the peripheral blood of acute stroke patients or asymptomatic individuals with vascular risk factors by fluorescence or magnetic activated cell sorting with anti-PDGF receptor-beta (PDGFR) antibody. The morphologic and molecular features of circulating PDGFR beta(+) cells were compared with tissue pericytes, and the associations with respect to quantity in the blood, culture outcome, and patient characteristics were analyzed. We found an increase in circulating PDGFR beta(+) cells in acute stroke patients compared to controls and a correlation with neurologic impairment. The isolated PDGFR beta(+) cells expressed mesenchymal stem cell markers, proliferated, and were multipotent under permissive culture conditions. The multipotent nature of these cells was comparable to fat-derived PDGFR beta(+) cells. These cells could be obtained by pharmacologic stimulation using bone marrow mobilizer. Circulating PDGFR beta(+) cells will be useful for future research involving endogenous recovery or autologous cell-based therapy. (C) 2010 Elsevier Inc. All rights reserved.