Unconventional Salt Trend from Soft to Stiff in Single Neurofilament Biopolymers

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We present persistence length measurements on neurofilaments (NFs), an intermediate filament with protruding side arms, of the neuronal cytoskeleton. Tapping mode atomic force microscopy enabled us to visualize and trace at subpixel resolution photoimmobilized NFs, assembled at various subunit protein ratios, thereby modifying the side-arm length and chain density charge distribution. We show that specific polyampholyte sequences of the side arms can form salt-switchable intrafilament attractions that compete with the net electrostatic and steric repulsion and can reduce the total persistence length by half. The results are in agreement with present X-ray and microscopy data yet present a theoretical challenge for polyampholyte interchain interactions.
Publisher
AMER CHEMICAL SOC
Issue Date
2010-12
Language
English
Article Type
Article
Keywords

ELECTROSTATIC PERSISTENCE LENGTH; INTERMEDIATE-FILAMENTS; SEMIFLEXIBLE POLYMERS; AXONAL-TRANSPORT; IN-VITRO; DNA; BRUSH; POLYELECTROLYTE; NETWORKS; DISEASE

Citation

LANGMUIR, v.26, no.24, pp.18595 - 18599

ISSN
0743-7463
DOI
10.1021/la103655x
URI
http://hdl.handle.net/10203/98136
Appears in Collection
BiS-Journal Papers(저널논문)
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