Comparative proteomic and genetic analyses reveal unidentified mutations in Escherichia coli XL1-Blue and DH5 alpha

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Escherichia coli has been used widely in laboratory and the biotech industry. However, the genetic and metabolic characteristics remain inadequately studied, particularly for those strains with extensive genetic manipulations that might have resulted in unknown mutations. Here, we demonstrate a comparative proteomics and genetics approach to identify unknown mutations in E. coli K-12 derivatives. The comparative proteomic and genetic analyses revealed an IS5 disruption of the kdgR gene in two commonly used derivative strains of E. coli K-12, XL1-Blue and DH5 alpha, compared with K-12 wild-type strain W3110. In addition, a controversial deoR mutation was clarified as a wild type in E. coli DH5 alpha using the same approach. This approach should be useful in characterizing the unknown mutations in various mutant strains developed. At the same time, comparative proteomic analysis also revealed the distinct metabolic characteristic of the two derivatives: higher biosynthetic flux to purine nucleotides. This is potentially beneficial for the synthesis of plasmid DNA.
Publisher
WILEY-BLACKWELL PUBLISHING
Issue Date
2011-01
Language
English
Article Type
Article
Keywords

SYSTEMS; METABOLISM; ACTIVATION; TRANSPORT; MUTANTS; BIOLOGY; GROWTH; STRAIN

Citation

FEMS MICROBIOLOGY LETTERS, v.314, no.2, pp.119 - 124

ISSN
0378-1097
DOI
10.1111/j.1574-6968.2010.02157.x
URI
http://hdl.handle.net/10203/98129
Appears in Collection
CBE-Journal Papers(저널논문)
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