DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seo, Moon-Hyeong | ko |
dc.contributor.author | Lee, Tae-Sun | ko |
dc.contributor.author | Kim, Eun-Kyung | ko |
dc.contributor.author | Cho, Young-Lag | ko |
dc.contributor.author | Park, Hee-Sung | ko |
dc.contributor.author | Yoon, Tae-Young | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2013-03-10T23:44:59Z | - |
dc.date.available | 2013-03-10T23:44:59Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v.83, no.23, pp.8849 - 8854 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97708 | - |
dc.description.abstract | Single-molecule fluorescence resonance energy transfer (smFRET) measurement provides a unique and powerful approach to understand complex biological processes including conformational and structural dynamics of individual biomolecules. For effective smFRET analysis of protein, site-specific dual-labeling with two fluorophores as an energy donor and an acceptor is crucial. Here we demonstrate that site-specific dual-labeling of protein via incorporation of unnatural amino acid provides a clearer picture for the folded and unfolded states of the protein in smFRET analysis than conventional labeling using double cysteines. As a model study, maltose-binding protein (MBP) was dually labeled via incorporation of rho-azido-L-phenylalanine and cysteine at specific positions, immobilized on a surface, and subjected to smFRET analysis under native and denaturing conditions. The resulting histograms show that site-specific dual-labeling results in a more homogeneous distribution in protein populations, enabling a precise smFRET analysis of protein. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | AZIDE-ALKYNE CYCLOADDITION | - |
dc.subject | CLICK CHEMISTRY | - |
dc.subject | FRET | - |
dc.title | Efficient Single-Molecule Fluorescence Resonance Energy Transfer Analysis by Site-Specific Dual-Labeling of Protein Using an Unnatural Amino Acid | - |
dc.type | Article | - |
dc.identifier.wosid | 000297481700004 | - |
dc.identifier.scopusid | 2-s2.0-82555168085 | - |
dc.type.rims | ART | - |
dc.citation.volume | 83 | - |
dc.citation.issue | 23 | - |
dc.citation.beginningpage | 8849 | - |
dc.citation.endingpage | 8854 | - |
dc.citation.publicationname | ANALYTICAL CHEMISTRY | - |
dc.contributor.localauthor | Park, Hee-Sung | - |
dc.contributor.localauthor | Yoon, Tae-Young | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Cho, Young-Lag | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | AZIDE-ALKYNE CYCLOADDITION | - |
dc.subject.keywordPlus | CLICK CHEMISTRY | - |
dc.subject.keywordPlus | FRET | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.