RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells

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RhoB, a tumor suppressor, has emerged as an interesting cancer target, and extensive studies aimed at understanding its role in apoptosis have been performed. In our study, we investigated the involvement of RhoB-interacting molecules in apoptosis. To identify RhoB-interacting proteins, we performed yeast-two hybrid screening assays using RhoB as a bait and isolated TNFAIP1, a TNF alpha-induced protein containing the BTB/POZ domain. The interaction between RhoB and TNFAIP1 was demonstrated in vivo through coimmunoprecipitation studies and in vitro binding assays. RFP-TNFAIP1 was found to be partially colocalized with EGFP-RhoB. The partial colocalization of Rill and TNFAIP1 in endosomes suggests that RhoB-TNFAIP1 interactions may have a functional role in apoptosis. TNFAIP1 elicited proapoptotic activity, while simultaneous expression of RhoB and TNFAIP1 resulted in a dramatic increase in apoptosis in HeLa cells. Furthermore, knockdown of RhoB using siRNA clearly rescued cells from apoptosis induced by TNFAIP1. This finding suggests that interactions between RhoB and TNFAIP1 are crucial for induction of apoptosis in HeLa cells. The observation of increased SAPK/JNK phosphorylation in apoptotic cells and the finding that a JNK inhibitor suppressed apoptosis indicates that SAPK/JNK signaling may be involved in apoptosis induced by RhoB-TNFAIP1 interactions. In conclusion, we found that RhoB interacts with TNFAIP1 to regulate apoptosis via a SAPK/JNK-mediated signal transduction mechanism. (c) 2009 UICC
Publisher
WILEY-LISS
Issue Date
2009-12
Language
English
Article Type
Article
Keywords

FARNESYL TRANSFERASE INHIBITION; ACTIN CYTOSKELETON; FARNESYLTRANSFERASE INHIBITORS; POTASSIUM CHANNEL; PROTEIN; TRANSFORMATION; DOMAIN; GENE; EXPRESSION; CANCER

Citation

INTERNATIONAL JOURNAL OF CANCER, v.125, no.11, pp.2520 - 2527

ISSN
0020-7136
DOI
10.1002/ijc.24617
URI
http://hdl.handle.net/10203/97466
Appears in Collection
BS-Journal Papers(저널논문)
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