DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Hyun Jung | ko |
dc.contributor.author | Kim, Mi Ju | ko |
dc.contributor.author | Song, Su Jung | ko |
dc.contributor.author | Kim, Tackhoon | ko |
dc.contributor.author | Lee, Dongjun | ko |
dc.contributor.author | Kwon, Seung-Hae | ko |
dc.contributor.author | Choi, Eui-Ju | ko |
dc.contributor.author | Lim, Dae-Sik | ko |
dc.date.accessioned | 2013-03-09T20:59:27Z | - |
dc.date.available | 2013-03-09T20:59:27Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-03 | - |
dc.identifier.citation | CURRENT BIOLOGY, v.20, no.5, pp.416 - 422 | - |
dc.identifier.issn | 0960-9822 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97445 | - |
dc.description.abstract | The establishment and maintenance of proper attachment of kinetochores to microtubules are required to prevent chromosome missegregation and consequent chromosomal instability and tumorigenesis. Although MST1 (mammalian sterile 20-like kinase 1) has been implicated in many aspects of cell cycle regulation and tumor suppression [1], its precise mechanism of action has remained largely unknown. We now show that MST1 promotes accurate kinetochore-microtubule attachment by modulating the kinase activity of Aurora B. HeLa cells depleted of MST1 failed to develop stable end-on kinetochore-microtubule attachment, giving rise to unaligned mitotic chromosomes. The misaligned chromosomes activated the Mad2- and BubR1-dependent spindle checkpoint response, resulting in a delay in anaphase onset. The kinase activity of Aurora B, which promotes destabilization of kinetochore-microtubule attachment [2-4], was increased in cells depleted of MST1 or NDR1, a downstream kinase of MST1. MST1 and NDR1 associated with Aurora B. Moreover, MST1 directly phosphorylated Aurora B and inhibited its kinase activity in vitro. Depletion of Aurora B restored the stability of kinetochore-microtubule attachment in cells depleted of MST1 or NDR1. MST1 is thus a key regulator of Aurora B activity that ensures mitotic chromosome congression and accurate chromosome segregation. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.subject | CHROMOSOME-SPINDLE ATTACHMENTS | - |
dc.subject | SMALL-MOLECULE INHIBITOR | - |
dc.subject | TUMOR-SUPPRESSOR | - |
dc.subject | CELL-PROLIFERATION | - |
dc.subject | APOPTOSIS | - |
dc.subject | ORIENTATION | - |
dc.subject | CHECKPOINT | - |
dc.subject | STABILITY | - |
dc.subject | DYNAMICS | - |
dc.subject | COMPLEX | - |
dc.title | MST1 Limits the Kinase Activity of Aurora B to Promote Stable Kinetochore-Microtubule Attachment | - |
dc.type | Article | - |
dc.identifier.wosid | 000275476900021 | - |
dc.identifier.scopusid | 2-s2.0-77649183891 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 416 | - |
dc.citation.endingpage | 422 | - |
dc.citation.publicationname | CURRENT BIOLOGY | - |
dc.identifier.doi | 10.1016/j.cub.2009.12.054 | - |
dc.contributor.localauthor | Lim, Dae-Sik | - |
dc.contributor.nonIdAuthor | Kwon, Seung-Hae | - |
dc.contributor.nonIdAuthor | Choi, Eui-Ju | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CELLBIO | - |
dc.subject.keywordPlus | CHROMOSOME-SPINDLE ATTACHMENTS | - |
dc.subject.keywordPlus | SMALL-MOLECULE INHIBITOR | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR | - |
dc.subject.keywordPlus | CELL-PROLIFERATION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | ORIENTATION | - |
dc.subject.keywordPlus | CHECKPOINT | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | COMPLEX | - |
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