DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chae, Han Seung | ko |
dc.contributor.author | Kim, Kong-Hwan | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.contributor.author | Lee, Pyung Cheon | ko |
dc.date.accessioned | 2013-03-09T20:13:31Z | - |
dc.date.available | 2013-03-09T20:13:31Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-12 | - |
dc.identifier.citation | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.162, no.8, pp.2333 - 2344 | - |
dc.identifier.issn | 0273-2289 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97365 | - |
dc.description.abstract | Seven Escherichia coli strains, which were metabolically engineered with carotenoid biosynthetic pathways, were systematically compared in order to investigate the strain-specific formation of carotenoids of structural diversity. C30 acyclic carotenoids, diaponeurosporene and diapolycopene were well produced in all E. coli strains tested. However, the C30 monocyclic diapotorulene formation was strongly strain dependent. Reduced diapotorulene formation was observed in the E. coli strain Top 10, MG1655, and MDS42 while better formation was observed in the E. coli strain JM109, SURE, DH5a, and XL1-Blue. Interestingly, C40 carotenoids, which have longer backbones than C30 carotenoids, also showed strain dependency as C30 diapotorulene did. Quantitative analysis showed that the SURE strain was the best producer for C40 acyclic lycopene, C40 dicyclic beta-carotene, and C30 monocyclic diapotorulene. Of the seven strains examined, the highest volumetric productivity for most of the carotenoids structures was observed in the recombinant SURE strain. In conclusion, we showed that recombinant hosts and carotenoid structures influenced carotenoid productions significantly, and this information can serve as the basis for the subsequent development of microorganisms for carotenoids of interest. | - |
dc.language | English | - |
dc.publisher | HUMANA PRESS INC | - |
dc.subject | LYCOPENE PRODUCTION | - |
dc.subject | ISOPENTENYL DIPHOSPHATE | - |
dc.subject | DIRECTED EVOLUTION | - |
dc.subject | PATHWAY | - |
dc.subject | COMBINATORIAL | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | MEVALONATE | - |
dc.subject | DIVERSITY | - |
dc.subject | ENZYMES | - |
dc.title | Strain-Dependent Carotenoid Productions in Metabolically Engineered Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000284295000019 | - |
dc.identifier.scopusid | 2-s2.0-78149465758 | - |
dc.type.rims | ART | - |
dc.citation.volume | 162 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 2333 | - |
dc.citation.endingpage | 2344 | - |
dc.citation.publicationname | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Chae, Han Seung | - |
dc.contributor.nonIdAuthor | Kim, Kong-Hwan | - |
dc.contributor.nonIdAuthor | Lee, Pyung Cheon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Carotenoids | - |
dc.subject.keywordAuthor | E. coli | - |
dc.subject.keywordAuthor | Strain dependency | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Pathway engineering | - |
dc.subject.keywordPlus | LYCOPENE PRODUCTION | - |
dc.subject.keywordPlus | ISOPENTENYL DIPHOSPHATE | - |
dc.subject.keywordPlus | DIRECTED EVOLUTION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | COMBINATORIAL | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | MEVALONATE | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | ENZYMES | - |
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