DC Field | Value | Language |
---|---|---|
dc.contributor.author | Conrad, Tom M. | ko |
dc.contributor.author | Frazier, Michael | ko |
dc.contributor.author | Joyce, Andrew R. | ko |
dc.contributor.author | Cho, Byung-Kwan | ko |
dc.contributor.author | Knight, Eric M. | ko |
dc.contributor.author | Lewis, Nathan E. | ko |
dc.contributor.author | Landick, Robert | ko |
dc.contributor.author | Palsson, Bernhard O. | ko |
dc.date.accessioned | 2013-03-09T20:00:46Z | - |
dc.date.available | 2013-03-09T20:00:46Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-11 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.107, no.47, pp.20500 - 20505 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10203/97350 | - |
dc.description.abstract | Specific small deletions within the rpoC gene encoding the beta'-subunit of RNA polymerase (RNAP) are found repeatedly after adaptation of Escherichia coli K-12 MG1655 to growth in minimal media. Here we present a multiscale analysis of these mutations. At the physiological level, the mutants grow 60% faster than the parent strain and convert the carbon source 15-35% more efficiently to biomass, but grow about 30% slower than the parent strain in rich medium. At the molecular level, the kinetic parameters of the mutated RNAP were found to be altered, resulting in a 4- to 30-fold decrease in open complex longevity at an rRNA promoter and a similar to 10-fold decrease in transcriptional pausing, with consequent increase in transcript elongation rate. At a genome-scale, systems biology level, gene expression changes between the parent strain and adapted RNAP mutants reveal large-scale systematic transcriptional changes that influence specific cellular processes, including strong down-regulation of motility, acid resistance, fimbria, and curlin genes. RNAP genome-binding maps reveal redistribution of RNAP that may facilitate relief of a metabolic bottleneck to growth. These findings suggest that reprogramming the kinetic parameters of RNAP through specific mutations allows regulatory adaptation for optimal growth in new environments. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | AMINO-ACID STARVATION | - |
dc.subject | TRANSCRIPTION ELONGATION | - |
dc.subject | STRINGENT RESPONSE | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | RPOB MUTANTS | - |
dc.subject | ARRAY DATA | - |
dc.subject | MUTATIONS | - |
dc.subject | PPGPP | - |
dc.subject | TERMINATION | - |
dc.subject | INITIATION | - |
dc.title | RNA polymerase mutants found through adaptive evolution reprogram Escherichia coli for optimal growth in minimal media | - |
dc.type | Article | - |
dc.identifier.wosid | 000284529000065 | - |
dc.identifier.scopusid | 2-s2.0-78650546051 | - |
dc.type.rims | ART | - |
dc.citation.volume | 107 | - |
dc.citation.issue | 47 | - |
dc.citation.beginningpage | 20500 | - |
dc.citation.endingpage | 20505 | - |
dc.citation.publicationname | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.identifier.doi | 10.1073/pnas.0911253107 | - |
dc.contributor.localauthor | Cho, Byung-Kwan | - |
dc.contributor.nonIdAuthor | Conrad, Tom M. | - |
dc.contributor.nonIdAuthor | Frazier, Michael | - |
dc.contributor.nonIdAuthor | Joyce, Andrew R. | - |
dc.contributor.nonIdAuthor | Knight, Eric M. | - |
dc.contributor.nonIdAuthor | Lewis, Nathan E. | - |
dc.contributor.nonIdAuthor | Landick, Robert | - |
dc.contributor.nonIdAuthor | Palsson, Bernhard O. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | kinetics | - |
dc.subject.keywordAuthor | stringent response | - |
dc.subject.keywordAuthor | transcription | - |
dc.subject.keywordPlus | AMINO-ACID STARVATION | - |
dc.subject.keywordPlus | TRANSCRIPTION ELONGATION | - |
dc.subject.keywordPlus | STRINGENT RESPONSE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | RPOB MUTANTS | - |
dc.subject.keywordPlus | ARRAY DATA | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | PPGPP | - |
dc.subject.keywordPlus | TERMINATION | - |
dc.subject.keywordPlus | INITIATION | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.